Structure and polymorphism of the upstream region of the pfg27/25 gene, transcriptionally regulated in gametocytogenesis of Plasmodium falciparum
Transcription of the early gametocyte-specific gene pfg27/25, and genomic structure and polymorphism of its upstream region were studied in the human malaria parasite Plasmodium falciparum. The upstream genomic sequence of the pfg27/25 gene is characterised by a repetitive region that contains five...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1996-08, Vol.79 (2), p.207-217 |
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| creator | Alano, P Silvestrini, F Roca, L |
| description | Transcription of the early gametocyte-specific gene pfg27/25, and genomic structure and polymorphism of its upstream region were studied in the human malaria parasite Plasmodium falciparum. The upstream genomic sequence of the pfg27/25 gene is characterised by a repetitive region that contains five direct and one inverted repeats of a unit constituted by a perfectly conserved sequence flanked by a poly-dT and a poly-d(AT) tract. Sequences further upstream from the repetitive region are polymorphic in distantly related parasite lines. Nuclear 'run off' experiments indicated that transcription of pfg27/25 is developmentally regulated. Transcription of the gene, undetectable in asexual parasites, is activated at the onset of gametocytogenesis. Te gene is transcribed in a 2.5 kb mature mRNA for the first 2-3 days of sexual differentiation, while transcription is down-regulated in more mature gametocytes. |
| doi_str_mv | 10.1016/0166-6851(96)02663-1 |
| format | Article |
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The upstream genomic sequence of the pfg27/25 gene is characterised by a repetitive region that contains five direct and one inverted repeats of a unit constituted by a perfectly conserved sequence flanked by a poly-dT and a poly-d(AT) tract. Sequences further upstream from the repetitive region are polymorphic in distantly related parasite lines. Nuclear 'run off' experiments indicated that transcription of pfg27/25 is developmentally regulated. Transcription of the gene, undetectable in asexual parasites, is activated at the onset of gametocytogenesis. Te gene is transcribed in a 2.5 kb mature mRNA for the first 2-3 days of sexual differentiation, while transcription is down-regulated in more mature gametocytes.</description><identifier>ISSN: 0166-6851</identifier><identifier>DOI: 10.1016/0166-6851(96)02663-1</identifier><identifier>PMID: 8855557</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Base Sequence ; Cloning, Molecular ; Gametogenesis - genetics ; Genes, Protozoan - genetics ; Molecular Sequence Data ; Plasmodium falciparum ; Plasmodium falciparum - genetics ; Plasmodium falciparum - growth & development ; Polymorphism, Genetic ; Protozoan Proteins - genetics ; Transcription, Genetic</subject><ispartof>Molecular and biochemical parasitology, 1996-08, Vol.79 (2), p.207-217</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8855557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alano, P</creatorcontrib><creatorcontrib>Silvestrini, F</creatorcontrib><creatorcontrib>Roca, L</creatorcontrib><title>Structure and polymorphism of the upstream region of the pfg27/25 gene, transcriptionally regulated in gametocytogenesis of Plasmodium falciparum</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>Transcription of the early gametocyte-specific gene pfg27/25, and genomic structure and polymorphism of its upstream region were studied in the human malaria parasite Plasmodium falciparum. 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Te gene is transcribed in a 2.5 kb mature mRNA for the first 2-3 days of sexual differentiation, while transcription is down-regulated in more mature gametocytes.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Gametogenesis - genetics</subject><subject>Genes, Protozoan - genetics</subject><subject>Molecular Sequence Data</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - genetics</subject><subject>Plasmodium falciparum - growth & development</subject><subject>Polymorphism, Genetic</subject><subject>Protozoan Proteins - genetics</subject><subject>Transcription, Genetic</subject><issn>0166-6851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EtLAzEQAOAclFqr_0AhJ1Fw7WazyWaPUnxBQUE9l2webWSziXkc-jP8x26xenVgGGb4GJgB4AyVN6hEdD4mLSgj6LKlV2VFKS7QAZj-jY_AcYwfZVmShtIJmDBGxmim4Os1hSxSDgryQULv-q11wW9MtNBpmDYKZh9TUNzCoNbGDb9jr9dVM68IXKtBXcMU-BBFMD6Nhvf9dsdzz5OS0Axwza1KTmyT2_Fo4m7NS8-jddJkCzXvhfE8ZHsCDscmqtN9nYH3-7u3xWOxfH54WtwuC49IlYqu5bqTCCGtW8JEoyWjtdTteHrHJcaKciEQohoT3IiqLWtcIyaJlLzrWMvwDFz87PXBfWYV08qaKFTf80G5HFcNq1GNWf0vRISOFJERnu9h7qySKx-M5WG72j8bfwOtg4OW</recordid><startdate>199608</startdate><enddate>199608</enddate><creator>Alano, P</creator><creator>Silvestrini, F</creator><creator>Roca, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199608</creationdate><title>Structure and polymorphism of the upstream region of the pfg27/25 gene, transcriptionally regulated in gametocytogenesis of Plasmodium falciparum</title><author>Alano, P ; Silvestrini, F ; Roca, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p152t-b9afbd111ff958c7fd864df9663bad33e6acc116f3537c29043418d5ddabb8983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Gametogenesis - genetics</topic><topic>Genes, Protozoan - genetics</topic><topic>Molecular Sequence Data</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - genetics</topic><topic>Plasmodium falciparum - growth & development</topic><topic>Polymorphism, Genetic</topic><topic>Protozoan Proteins - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alano, P</creatorcontrib><creatorcontrib>Silvestrini, F</creatorcontrib><creatorcontrib>Roca, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alano, P</au><au>Silvestrini, F</au><au>Roca, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure and polymorphism of the upstream region of the pfg27/25 gene, transcriptionally regulated in gametocytogenesis of Plasmodium falciparum</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1996-08</date><risdate>1996</risdate><volume>79</volume><issue>2</issue><spage>207</spage><epage>217</epage><pages>207-217</pages><issn>0166-6851</issn><abstract>Transcription of the early gametocyte-specific gene pfg27/25, and genomic structure and polymorphism of its upstream region were studied in the human malaria parasite Plasmodium falciparum. 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| ispartof | Molecular and biochemical parasitology, 1996-08, Vol.79 (2), p.207-217 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Base Sequence Cloning, Molecular Gametogenesis - genetics Genes, Protozoan - genetics Molecular Sequence Data Plasmodium falciparum Plasmodium falciparum - genetics Plasmodium falciparum - growth & development Polymorphism, Genetic Protozoan Proteins - genetics Transcription, Genetic |
| title | Structure and polymorphism of the upstream region of the pfg27/25 gene, transcriptionally regulated in gametocytogenesis of Plasmodium falciparum |
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