Expression of hepatitis A virus capsid sequences in insect cells

Expression of hepatitis A virus capsid sequences in insect cells

A cDNA coding for hepatitis A virus (HAV) VP1 and portions of the flanking VP3 and P2 sequences was inserted into the genome of Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter and translational start codon. Cells infected with recombinant virus produced...

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Veröffentlicht in:Virus research 1988-05, Vol.10 (2), p.273-280
Hauptverfasser: Harmon, Shirley A., Johnston, Jeffrey M., Ziegelhoffer, Thomas, Richards, Oliver C., Summers, Donald F., Ehrenfeld, Ellie
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Autographa californica
Baculovirus vector
Biological and medical sciences
Capsid - biosynthesis
Capsid - genetics
Capsid protein expression
Clone Cells
DNA Restriction Enzymes
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genetic Vectors
Genetics
Hepatitis A virus
Hepatovirus - genetics
Humans
Immunoassay
Insect Viruses - genetics
Microbiology
Moths
nuclear polyhedrosis virus
Plasmids
Promoter Regions, Genetic
Rabbits
Virology
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Zusammenfassung:A cDNA coding for hepatitis A virus (HAV) VP1 and portions of the flanking VP3 and P2 sequences was inserted into the genome of Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter and translational start codon. Cells infected with recombinant virus produced high levels of a 55 kDa protein, identified as containing HAV VP1 by reactivity with anti-VP1 serum. The expressed protein also reacted on immunoblots with human HAV convalescent sera as well as sera from rabbits immunized with intact HAV. This protein was found predominantly in the cytoplasm of infected insect cells, probably as an insoluble aggregate.
ISSN:0168-1702
1872-7492
DOI:10.1016/0168-1702(88)90022-6