Enalaprilat, an Angiotensin-converting Enzyme Inhibitor, Enhances Functional Preservation During Long-term Cardiac Preservation. Possible Involvement of Bradykinin and PKC

We investigated the effect of enalaprilat (ET) on long-term preservation of cardiac explant. Isolated rat hearts ( n=7/group) were pretreated with 0 to 40 n mET and stored at 0°C for 16 h. Functional viability was assessed after 30-min working reperfusion without ET. Prestorage control function (mean± s.e.m.) ( n=7) included heart rate (HR), 291±12 bpm; aortic flow (AF), 49.8±1.9 ml/min; coronary flow (CF), 25.2±1.3 ml/min; cardiac output (CO), 75.0±1.2 ml/min; and work, 89.4±4.7 g-m/min. Post-storage function of untreated hearts was: AF, 61%; CF, 43%; CO, 55%; work, 48% of control. ET (20 n m) enhanced AF recovery to 85%; CF, 58%; CO, 76%; work, 73% of control ( P