Expression of varicella-zoster virus glycoprotein I in cells infected with a vaccinia virus recombinant

BSC-1 cells infected with a vaccinia virus recombinant containing the coding sequences for varicella-zoster virus (VZV) glycoprotein I (gpI) were analyzed by indirect immunofluorescence and immunoprecipitation for the expression and processing of gpI. The processing of gpI in cells infected with rec...

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Veröffentlicht in:	Virus research 1988-05, Vol.10 (2), p.205-213
Hauptverfasser:	Cabirac, Gary, Gilden, Donald, Wellish, Mary, Vafai, Abbas
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Line Cloning, Molecular Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genetic Vectors Genetics Glycoprotein I Herpesvirus 3, Human - genetics Immunoassay Microbiology Recombinant vaccinia vaccinia virus Vaccinia virus - genetics Varicella-zoster virus Viral Envelope Proteins Viral Proteins - biosynthesis Viral Proteins - genetics Virology
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Beschreibung
Zusammenfassung:	BSC-1 cells infected with a vaccinia virus recombinant containing the coding sequences for varicella-zoster virus (VZV) glycoprotein I (gpI) were analyzed by indirect immunofluorescence and immunoprecipitation for the expression and processing of gpI. The processing of gpI in cells infected with recombinant virus was the same as that observed during VZV infection. Immunofluorescence revealed localization of gpI to the membranes of recombinant virus-infected cells.
ISSN:	0168-1702 1872-7492
DOI:	10.1016/0168-1702(88)90016-0