Expression of Theiler's murine encephalomyelitis virus protease 3C and polymerase 3D in Escherichia coli and characterization of monospecific sera

Defined DNA fragments of cloned Theiler's murine encephalomyelitis virus genome were used to construct procaryotic recombinant plasmids expressing viral genes 3C and 3D. In these plasmids (pEX-EMBL vectors), viral sequences are fused in-phase behind the Escherichia coli lac Z′ gene which is und...

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Veröffentlicht in:	Virology (New York, N.Y.) N.Y.), 1988-08, Vol.165 (2), p.596-600
Hauptverfasser:	Ozden, Simona, Aubert, Christine, Brahic, Michel
Format:	Artikel
Sprache:	eng
Schlagworte:	3C Viral Proteases Animals Biological and medical sciences Cell Line Chemical Precipitation Cricetinae Cysteine Endopeptidases Endopeptidases - genetics Endopeptidases - immunology Enterovirus - genetics Fundamental and applied biological sciences. Psychology Immunoenzyme Techniques Immunosorbent Techniques Maus Elberfeld virus - enzymology Maus Elberfeld virus - genetics Maus Elberfeld virus - immunology Microbiology Peptide Hydrolases - genetics Peptide Hydrolases - immunology Recombinant Fusion Proteins - immunology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA Nucleotidyltransferases - genetics RNA-Dependent RNA Polymerase - genetics RNA-Dependent RNA Polymerase - immunology Viral Proteins Virology
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Zusammenfassung:	Defined DNA fragments of cloned Theiler's murine encephalomyelitis virus genome were used to construct procaryotic recombinant plasmids expressing viral genes 3C and 3D. In these plasmids (pEX-EMBL vectors), viral sequences are fused in-phase behind the Escherichia coli lac Z′ gene which is under the control of the inducible λ Pr promotor. Partially purified fusion proteins were used to immunize Balb/c mice. Sera monospecific for the viral protease 3C and polymerase 3D were obtained. These sera detected their corresponding antigens in situ in infected BHK cells using immunocytochemical reactions.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/0042-6822(88)90604-6