Molecular cloning and chromosomal localization of the human cyclin C (CCNC) and cyclin E (CCNE) genes : Deletion of the CCNC gene in human tumors

The human Gi-phase cyclins are important regulators of cell cycle progression that interact with various cyclin-dependent kinases and facilitate entry into S-phase. We have confirmed the localization of the human cyclin C (CCNC) gene to chromosome 6q21 and of human cyclin E (CCNE) to 19q12. The CCNC...

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Veröffentlicht in:Genomics 1996-03, Vol.32 (2), p.253-259
Hauptverfasser: HAIMIN LI, LAHTI, J. M, VALENTINE, M, SAITO, M, REED, S. I, LOOK, A. T, KIDD, V. J
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Sprache:eng
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Zusammenfassung:The human Gi-phase cyclins are important regulators of cell cycle progression that interact with various cyclin-dependent kinases and facilitate entry into S-phase. We have confirmed the localization of the human cyclin C (CCNC) gene to chromosome 6q21 and of human cyclin E (CCNE) to 19q12. The CCNC gene structure was also determined, and we have shown that it is deleted in a subset of acute lymphoblastic leukemias, including a patient sample containing a t(2;6)(p21;q15), with no apparent cytogenetic deletion. Single-strand conformational polymorphism analysis of the remaining CCNC allele from patients with a deletion of one allele established that there were no further mutations within the exons or the flanking intronic sequences. These results suggest either that haploinsufficiency of the cyclin C protein is sufficient to promote tumorigenesis or that the important tumor suppressor gene is linked to the CCNC locus.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0112