Sequence and genomic organization of GBV-C: A novel member of the flaviviridae associated with human non-A-E hepatitis

Recently, sequences from a novel virus, termed GB virus C (GBV‐C), were identified in serum from several patients with cryptogenic hepatitis. In the present study, the nucleotide sequence of this virus has been extended to near‐genome length. GBV‐C encodes a putative single large polyprotein in which the structural proteins are positioned at the N‐terminal end, with the nonstructural proteins located at the C‐terminal end. Amino acid sequence analysis of this large polyprotein reveals the presence of protease, helicase, and replicase motifs. Sequence alignments of the polyprotein followed by phylogenetic analyses suggest that GBV‐C is a member of the Flaviviridae, most closely related to the recently described GB virus A. © 1996 Wiley‐Liss, Inc.