Molecular Cloning of a Genomic DNA for Enolase from Aspergillus oryzae

We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5′- and 3′- flanking regions. The homology between A. oryza...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996-01, Vol.60 (1), p.161-163
Hauptverfasser:	Machida, Masayuki, Violeta Jime'nez Gonzalez, Trini, Kong Boon, Lim, Gomi, Katsuya, Jigami, Yoshifumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Aspergillus oryzae Aspergillus oryzae - enzymology Base Sequence Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Blotting, Southern Cloning, Molecular DNA, Bacterial - chemistry Electrophoresis, Polyacrylamide Gel enolase Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic - genetics Genetics genomic DNA Mission oriented research Molecular Sequence Data Nucleic Acid Hybridization Phosphopyruvate Hydratase - chemistry Phosphopyruvate Hydratase - genetics Restriction Mapping TATA Box
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Zusammenfassung:	We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5′- and 3′- flanking regions. The homology between A. oryzae enoA and S. cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A. oryzae.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.60.161