Crystallization of a mammalian ornithine decarboxylase

Crystals of truncated (Δ425‐461) pyridoxal‐5′‐phosphate (PLP)‐dependent mouse ornithine decarboxylase (mOrnDC′) have been obtained that diffract to 2.2 Å resolution (P21212, a = 119.5 Å, b = 74.3 Å, c = 46.1 Å). OrnDC produces putrescine, which is the precursor for the synthesis of polyamines in euk...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proteins, structure, function, and bioinformatics structure, function, and bioinformatics, 1996-02, Vol.24 (2), p.266-268
Hauptverfasser:	Kern, Andrew, Oliveira, Marcos A., Chang, Ning-Leh, Ernst, Stephen R., Carroll, Donald W., Momany, Cory, Minard, Karyl, Coffino, Philip, Hackert, Marvin L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Crystallography, X-Ray Eflornithine - analogs & derivatives Eflornithine - chemistry Enzyme Inhibitors - chemistry group IV decarboxylases Mice Molecular Sequence Data Ornithine Decarboxylase - chemistry Ornithine Decarboxylase Inhibitors Peptide Fragments - chemistry polyamines pyridoxal phosphate Pyridoxal Phosphate - chemistry Sequence Deletion
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Crystals of truncated (Δ425‐461) pyridoxal‐5′‐phosphate (PLP)‐dependent mouse ornithine decarboxylase (mOrnDC′) have been obtained that diffract to 2.2 Å resolution (P21212, a = 119.5 Å, b = 74.3 Å, c = 46.1 Å). OrnDC produces putrescine, which is the precursor for the synthesis of polyamines in eukaryotes. Regulation of activity and understanding of the mechanism of action of this enzyme may aid in the development of compounds against cancer. mOrnDC is a member of group IV PLP‐dependent decarboxylases, for which there are no known representative structures.
ISSN:	0887-3585 1097-0134
DOI:	10.1002/(SICI)1097-0134(199602)24:2<266::AID-PROT15>3.0.CO;2-L