Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: Cloning of a second functional gene and identification of the Rt6 gene products

RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen. It has attracted interest as an activation antigen and because defective RT6-expression coincides with increased susceptibility for autoimmune type I diabetes in...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular immunology 1996-06, Vol.33 (9), p.807-817
Hauptverfasser: Hollmann, Christiane, Haag, Friedrich, Schlott, Martin, Damaske, André, Bertuleit, Heinrich, Matthes, Martina, Kühl, Maren, Thiele, Heinz-Günter, Koch-Nolte, Friedrich
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 817
container_issue 9
container_start_page 807
container_title Molecular immunology
container_volume 33
creator Hollmann, Christiane
Haag, Friedrich
Schlott, Martin
Damaske, André
Bertuleit, Heinrich
Matthes, Martina
Kühl, Maren
Thiele, Heinz-Günter
Koch-Nolte, Friedrich
description RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen. It has attracted interest as an activation antigen and because defective RT6-expression coincides with increased susceptibility for autoimmune type I diabetes in the BB rat. Southern blot analyses indicate that the rat carries a single copy RT6 gene whereas the mouse carries a duplication of the homologous locus. We had previously cloned and sequenced a RT6-homologous cDNA from BALB/c mouse spleen. We now report the cloning and characterization of a second RT6-homologue from BALB/c and 129/Sv mice. The two mouse Rt6 genes (designated Rt6-1 and Rt6-2) encode similar open reading frames that are disrupted by conserved introns. The nucleotide sequences of the Rt6-1 and Rt6-2 coding regions show 87% sequence identity, the deduced amino acid sequences 79% identity. The amino acid sequences reveal significant similarity to recently cloned ADP-ribosylating ectoenzymes from rabbit and human skeletal muscle as well as chicken bone marrow cells. RT-PCR analyses reveal that the two Rt6 genes are differentially expressed in distinct inbred mouse strains and that their transcripts are properly processed .Western blot analyses demonstrate that the respective gene products are released from cells by treatment with PI-PLC. The results further show that both mouse Rt6 genes are translated into GPI-anchored cell surface molecules and that Rt6 gene expression is restricted to peripheral lymphoid tissues.
doi_str_mv 10.1016/0161-5890(96)00008-9
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78344439</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0161589096000089</els_id><sourcerecordid>78344439</sourcerecordid><originalsourceid>FETCH-LOGICAL-c388t-385bdb68b557d34110d2ff919fe83e5165920ed29920feb57f02ab26b3793113</originalsourceid><addsrcrecordid>eNqFkc-OFCEQxonRrOPqG2jCyegBhaabBg-bbMa_yRqNmTuhodjF9MAKtMn6Kr6stDOZo5KQCtSvvkrVh9BTRl8xysTrdhkZpKIvlHhJ25FE3UMbJseOKNZ399HmhDxEj0r53hhBxXCGzqRkjI5ig35_TjPYZTYZ2xuTja2Qwy9TQ4o4ebxPSwG8IxbmGYOtiVy-_UpymFK5m2s2sXjIpiHfqniDt3OKIV6vhQYXsCk67JdoVzUz42uIgE37Cw5iDT7YU59681figNzm5BZby2P0wJu5wJNjPEe79-9224_k6suHT9vLK2K5lJVwOUxuEnIahtHxvg3mOu8VUx4kh4GJQXUUXKda8DANo6edmTox8VFxxvg5en6QbX1_LFCq3oeyDmwitPH1KHnf91z9F2TDOLYFdw3sD6DNqZQMXt_msDf5TjOqV-_0aoxejdGqPVbv9Kr_7Ki_THtwp6KjWS1_cchDW8bPAFkXGyBacCE3b7RL4d8N_gBmCam0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15770602</pqid></control><display><type>article</type><title>Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: Cloning of a second functional gene and identification of the Rt6 gene products</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Hollmann, Christiane ; Haag, Friedrich ; Schlott, Martin ; Damaske, André ; Bertuleit, Heinrich ; Matthes, Martina ; Kühl, Maren ; Thiele, Heinz-Günter ; Koch-Nolte, Friedrich</creator><creatorcontrib>Hollmann, Christiane ; Haag, Friedrich ; Schlott, Martin ; Damaske, André ; Bertuleit, Heinrich ; Matthes, Martina ; Kühl, Maren ; Thiele, Heinz-Günter ; Koch-Nolte, Friedrich</creatorcontrib><description>RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen. It has attracted interest as an activation antigen and because defective RT6-expression coincides with increased susceptibility for autoimmune type I diabetes in the BB rat. Southern blot analyses indicate that the rat carries a single copy RT6 gene whereas the mouse carries a duplication of the homologous locus. We had previously cloned and sequenced a RT6-homologous cDNA from BALB/c mouse spleen. We now report the cloning and characterization of a second RT6-homologue from BALB/c and 129/Sv mice. The two mouse Rt6 genes (designated Rt6-1 and Rt6-2) encode similar open reading frames that are disrupted by conserved introns. The nucleotide sequences of the Rt6-1 and Rt6-2 coding regions show 87% sequence identity, the deduced amino acid sequences 79% identity. The amino acid sequences reveal significant similarity to recently cloned ADP-ribosylating ectoenzymes from rabbit and human skeletal muscle as well as chicken bone marrow cells. RT-PCR analyses reveal that the two Rt6 genes are differentially expressed in distinct inbred mouse strains and that their transcripts are properly processed .Western blot analyses demonstrate that the respective gene products are released from cells by treatment with PI-PLC. The results further show that both mouse Rt6 genes are translated into GPI-anchored cell surface molecules and that Rt6 gene expression is restricted to peripheral lymphoid tissues.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/0161-5890(96)00008-9</identifier><identifier>PMID: 8811076</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>ADP-ribosyltransferase ; Amino Acid Sequence ; Animals ; Antigens, Differentiation, T-Lymphocyte - chemistry ; Antigens, Differentiation, T-Lymphocyte - genetics ; Antigens, Differentiation, T-Lymphocyte - isolation &amp; purification ; Base Sequence ; Blotting, Southern ; Cloning, Molecular ; Cross Reactions ; ectoenzyme ; gene-duplication ; GPI-anchorage ; Histocompatibility Antigens - chemistry ; Histocompatibility Antigens - genetics ; Histocompatibility Antigens - isolation &amp; purification ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Multigene Family - immunology ; Phosphatidylinositol Diacylglycerol-Lyase ; Phosphoinositide Phospholipase C ; Phosphoric Diester Hydrolases - pharmacology ; Poly(ADP-ribose) Polymerases - chemistry ; Poly(ADP-ribose) Polymerases - genetics ; Poly(ADP-ribose) Polymerases - isolation &amp; purification ; Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Spleen - immunology ; T cell marker Rt6 ; Transcription, Genetic - immunology</subject><ispartof>Molecular immunology, 1996-06, Vol.33 (9), p.807-817</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-385bdb68b557d34110d2ff919fe83e5165920ed29920feb57f02ab26b3793113</citedby><cites>FETCH-LOGICAL-c388t-385bdb68b557d34110d2ff919fe83e5165920ed29920feb57f02ab26b3793113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0161-5890(96)00008-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8811076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hollmann, Christiane</creatorcontrib><creatorcontrib>Haag, Friedrich</creatorcontrib><creatorcontrib>Schlott, Martin</creatorcontrib><creatorcontrib>Damaske, André</creatorcontrib><creatorcontrib>Bertuleit, Heinrich</creatorcontrib><creatorcontrib>Matthes, Martina</creatorcontrib><creatorcontrib>Kühl, Maren</creatorcontrib><creatorcontrib>Thiele, Heinz-Günter</creatorcontrib><creatorcontrib>Koch-Nolte, Friedrich</creatorcontrib><title>Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: Cloning of a second functional gene and identification of the Rt6 gene products</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen. It has attracted interest as an activation antigen and because defective RT6-expression coincides with increased susceptibility for autoimmune type I diabetes in the BB rat. Southern blot analyses indicate that the rat carries a single copy RT6 gene whereas the mouse carries a duplication of the homologous locus. We had previously cloned and sequenced a RT6-homologous cDNA from BALB/c mouse spleen. We now report the cloning and characterization of a second RT6-homologue from BALB/c and 129/Sv mice. The two mouse Rt6 genes (designated Rt6-1 and Rt6-2) encode similar open reading frames that are disrupted by conserved introns. The nucleotide sequences of the Rt6-1 and Rt6-2 coding regions show 87% sequence identity, the deduced amino acid sequences 79% identity. The amino acid sequences reveal significant similarity to recently cloned ADP-ribosylating ectoenzymes from rabbit and human skeletal muscle as well as chicken bone marrow cells. RT-PCR analyses reveal that the two Rt6 genes are differentially expressed in distinct inbred mouse strains and that their transcripts are properly processed .Western blot analyses demonstrate that the respective gene products are released from cells by treatment with PI-PLC. The results further show that both mouse Rt6 genes are translated into GPI-anchored cell surface molecules and that Rt6 gene expression is restricted to peripheral lymphoid tissues.</description><subject>ADP-ribosyltransferase</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antigens, Differentiation, T-Lymphocyte - chemistry</subject><subject>Antigens, Differentiation, T-Lymphocyte - genetics</subject><subject>Antigens, Differentiation, T-Lymphocyte - isolation &amp; purification</subject><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>Cross Reactions</subject><subject>ectoenzyme</subject><subject>gene-duplication</subject><subject>GPI-anchorage</subject><subject>Histocompatibility Antigens - chemistry</subject><subject>Histocompatibility Antigens - genetics</subject><subject>Histocompatibility Antigens - isolation &amp; purification</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family - immunology</subject><subject>Phosphatidylinositol Diacylglycerol-Lyase</subject><subject>Phosphoinositide Phospholipase C</subject><subject>Phosphoric Diester Hydrolases - pharmacology</subject><subject>Poly(ADP-ribose) Polymerases - chemistry</subject><subject>Poly(ADP-ribose) Polymerases - genetics</subject><subject>Poly(ADP-ribose) Polymerases - isolation &amp; purification</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spleen - immunology</subject><subject>T cell marker Rt6</subject><subject>Transcription, Genetic - immunology</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-OFCEQxonRrOPqG2jCyegBhaabBg-bbMa_yRqNmTuhodjF9MAKtMn6Kr6stDOZo5KQCtSvvkrVh9BTRl8xysTrdhkZpKIvlHhJ25FE3UMbJseOKNZ399HmhDxEj0r53hhBxXCGzqRkjI5ig35_TjPYZTYZ2xuTja2Qwy9TQ4o4ebxPSwG8IxbmGYOtiVy-_UpymFK5m2s2sXjIpiHfqniDt3OKIV6vhQYXsCk67JdoVzUz42uIgE37Cw5iDT7YU59681figNzm5BZby2P0wJu5wJNjPEe79-9224_k6suHT9vLK2K5lJVwOUxuEnIahtHxvg3mOu8VUx4kh4GJQXUUXKda8DANo6edmTox8VFxxvg5en6QbX1_LFCq3oeyDmwitPH1KHnf91z9F2TDOLYFdw3sD6DNqZQMXt_msDf5TjOqV-_0aoxejdGqPVbv9Kr_7Ki_THtwp6KjWS1_cchDW8bPAFkXGyBacCE3b7RL4d8N_gBmCam0</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Hollmann, Christiane</creator><creator>Haag, Friedrich</creator><creator>Schlott, Martin</creator><creator>Damaske, André</creator><creator>Bertuleit, Heinrich</creator><creator>Matthes, Martina</creator><creator>Kühl, Maren</creator><creator>Thiele, Heinz-Günter</creator><creator>Koch-Nolte, Friedrich</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19960601</creationdate><title>Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: Cloning of a second functional gene and identification of the Rt6 gene products</title><author>Hollmann, Christiane ; Haag, Friedrich ; Schlott, Martin ; Damaske, André ; Bertuleit, Heinrich ; Matthes, Martina ; Kühl, Maren ; Thiele, Heinz-Günter ; Koch-Nolte, Friedrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-385bdb68b557d34110d2ff919fe83e5165920ed29920feb57f02ab26b3793113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ADP-ribosyltransferase</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antigens, Differentiation, T-Lymphocyte - chemistry</topic><topic>Antigens, Differentiation, T-Lymphocyte - genetics</topic><topic>Antigens, Differentiation, T-Lymphocyte - isolation &amp; purification</topic><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>Cross Reactions</topic><topic>ectoenzyme</topic><topic>gene-duplication</topic><topic>GPI-anchorage</topic><topic>Histocompatibility Antigens - chemistry</topic><topic>Histocompatibility Antigens - genetics</topic><topic>Histocompatibility Antigens - isolation &amp; purification</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family - immunology</topic><topic>Phosphatidylinositol Diacylglycerol-Lyase</topic><topic>Phosphoinositide Phospholipase C</topic><topic>Phosphoric Diester Hydrolases - pharmacology</topic><topic>Poly(ADP-ribose) Polymerases - chemistry</topic><topic>Poly(ADP-ribose) Polymerases - genetics</topic><topic>Poly(ADP-ribose) Polymerases - isolation &amp; purification</topic><topic>Polymerase Chain Reaction</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spleen - immunology</topic><topic>T cell marker Rt6</topic><topic>Transcription, Genetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hollmann, Christiane</creatorcontrib><creatorcontrib>Haag, Friedrich</creatorcontrib><creatorcontrib>Schlott, Martin</creatorcontrib><creatorcontrib>Damaske, André</creatorcontrib><creatorcontrib>Bertuleit, Heinrich</creatorcontrib><creatorcontrib>Matthes, Martina</creatorcontrib><creatorcontrib>Kühl, Maren</creatorcontrib><creatorcontrib>Thiele, Heinz-Günter</creatorcontrib><creatorcontrib>Koch-Nolte, Friedrich</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hollmann, Christiane</au><au>Haag, Friedrich</au><au>Schlott, Martin</au><au>Damaske, André</au><au>Bertuleit, Heinrich</au><au>Matthes, Martina</au><au>Kühl, Maren</au><au>Thiele, Heinz-Günter</au><au>Koch-Nolte, Friedrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: Cloning of a second functional gene and identification of the Rt6 gene products</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>1996-06-01</date><risdate>1996</risdate><volume>33</volume><issue>9</issue><spage>807</spage><epage>817</epage><pages>807-817</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><abstract>RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen. It has attracted interest as an activation antigen and because defective RT6-expression coincides with increased susceptibility for autoimmune type I diabetes in the BB rat. Southern blot analyses indicate that the rat carries a single copy RT6 gene whereas the mouse carries a duplication of the homologous locus. We had previously cloned and sequenced a RT6-homologous cDNA from BALB/c mouse spleen. We now report the cloning and characterization of a second RT6-homologue from BALB/c and 129/Sv mice. The two mouse Rt6 genes (designated Rt6-1 and Rt6-2) encode similar open reading frames that are disrupted by conserved introns. The nucleotide sequences of the Rt6-1 and Rt6-2 coding regions show 87% sequence identity, the deduced amino acid sequences 79% identity. The amino acid sequences reveal significant similarity to recently cloned ADP-ribosylating ectoenzymes from rabbit and human skeletal muscle as well as chicken bone marrow cells. RT-PCR analyses reveal that the two Rt6 genes are differentially expressed in distinct inbred mouse strains and that their transcripts are properly processed .Western blot analyses demonstrate that the respective gene products are released from cells by treatment with PI-PLC. The results further show that both mouse Rt6 genes are translated into GPI-anchored cell surface molecules and that Rt6 gene expression is restricted to peripheral lymphoid tissues.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>8811076</pmid><doi>10.1016/0161-5890(96)00008-9</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0161-5890
ispartof Molecular immunology, 1996-06, Vol.33 (9), p.807-817
issn 0161-5890
1872-9142
language eng
recordid cdi_proquest_miscellaneous_78344439
source MEDLINE; Access via ScienceDirect (Elsevier)
subjects ADP-ribosyltransferase
Amino Acid Sequence
Animals
Antigens, Differentiation, T-Lymphocyte - chemistry
Antigens, Differentiation, T-Lymphocyte - genetics
Antigens, Differentiation, T-Lymphocyte - isolation & purification
Base Sequence
Blotting, Southern
Cloning, Molecular
Cross Reactions
ectoenzyme
gene-duplication
GPI-anchorage
Histocompatibility Antigens - chemistry
Histocompatibility Antigens - genetics
Histocompatibility Antigens - isolation & purification
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Multigene Family - immunology
Phosphatidylinositol Diacylglycerol-Lyase
Phosphoinositide Phospholipase C
Phosphoric Diester Hydrolases - pharmacology
Poly(ADP-ribose) Polymerases - chemistry
Poly(ADP-ribose) Polymerases - genetics
Poly(ADP-ribose) Polymerases - isolation & purification
Polymerase Chain Reaction
Sequence Homology, Amino Acid
Spleen - immunology
T cell marker Rt6
Transcription, Genetic - immunology
title Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: Cloning of a second functional gene and identification of the Rt6 gene products
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T23%3A50%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20characterization%20of%20mouse%20T-cell%20ecto-ADP-ribosyltransferase%20Rt6:%20Cloning%20of%20a%20second%20functional%20gene%20and%20identification%20of%20the%20Rt6%20gene%20products&rft.jtitle=Molecular%20immunology&rft.au=Hollmann,%20Christiane&rft.date=1996-06-01&rft.volume=33&rft.issue=9&rft.spage=807&rft.epage=817&rft.pages=807-817&rft.issn=0161-5890&rft.eissn=1872-9142&rft_id=info:doi/10.1016/0161-5890(96)00008-9&rft_dat=%3Cproquest_cross%3E78344439%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15770602&rft_id=info:pmid/8811076&rft_els_id=0161589096000089&rfr_iscdi=true