Molecular characterization of mouse T-cell ecto-ADP-ribosyltransferase Rt6: Cloning of a second functional gene and identification of the Rt6 gene products
RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen. It has attracted interest as an activation antigen and because defective RT6-expression coincides with increased susceptibility for autoimmune type I diabetes in...
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Veröffentlicht in: | Molecular immunology 1996-06, Vol.33 (9), p.807-817 |
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Sprache: | eng |
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Zusammenfassung: | RT6 is an enzymatically active GPI-anchored membrane protein that was originally discovered in the rat as a peripheral T cell alloantigen. It has attracted interest as an activation antigen and because defective RT6-expression coincides with increased susceptibility for autoimmune type I diabetes in the BB rat. Southern blot analyses indicate that the rat carries a single copy
RT6 gene whereas the mouse carries a duplication of the homologous locus. We had previously cloned and sequenced a
RT6-homologous cDNA from
BALB/c mouse spleen. We now report the cloning and characterization of a second
RT6-homologue from
BALB/c and
129/Sv mice. The two mouse
Rt6 genes (designated
Rt6-1 and
Rt6-2) encode similar open reading frames that are disrupted by conserved introns. The nucleotide sequences of the
Rt6-1 and
Rt6-2 coding regions show 87% sequence identity, the deduced amino acid sequences 79% identity. The amino acid sequences reveal significant similarity to recently cloned ADP-ribosylating ectoenzymes from rabbit and human skeletal muscle as well as chicken bone marrow cells. RT-PCR analyses reveal that the two
Rt6 genes are differentially expressed in distinct inbred mouse strains and that their transcripts are properly processed .Western blot analyses demonstrate that the respective gene products are released from cells by treatment with PI-PLC. The results further show that both mouse
Rt6 genes are translated into GPI-anchored cell surface molecules and that
Rt6 gene expression is restricted to peripheral lymphoid tissues. |
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ISSN: | 0161-5890 1872-9142 |
DOI: | 10.1016/0161-5890(96)00008-9 |