Partial amino acid sequence of rat topoisomerase I: Comparison with the predicted sequences for the human and yeast enzymes

The partial amino acid sequence of rat topoisomerase I was determined by gas-phase microsequencing. Seven tryptic peptides closely matched the sequences deduced from human topoisomerase I cDNA (94.5% homology). Similarity to sequences deduced from baker's yeast and fission yeast genomic DNA wer...

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Veröffentlicht in:	Biochemical and biophysical research communications 1988-07, Vol.154 (1), p.358-364
Hauptverfasser:	Durban, Egon, Bramucci, Massimo, Cook, Richard
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods DNA - genetics DNA Topoisomerases, Type I - genetics Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Humans Isomerases Liver Neoplasms, Experimental - enzymology Molecular Sequence Data Peptide Fragments - analysis Rats Saccharomyces cerevisiae - enzymology Schizosaccharomyces - enzymology Species Specificity Trypsin
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Zusammenfassung:	The partial amino acid sequence of rat topoisomerase I was determined by gas-phase microsequencing. Seven tryptic peptides closely matched the sequences deduced from human topoisomerase I cDNA (94.5% homology). Similarity to sequences deduced from baker's yeast and fission yeast genomic DNA were restricted to conserved domains which may represent important sites of interaction with DNA or with other proteins.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(88)90693-6