4-Hydroxy-2,3-trans-nonenal Induces Transcription and Expression of Aldose Reductase

Treatment of A7r5 rat vascular smooth muscle cells with 4-hydroxy-2,3-trans-nonenal (HNE) resulted in increased aldose reductase mRNA transcription. Induction was time dependent, reaching a maximum (2.3-fold) after 7 hours. An enzymatically active aldose reductase analysed by Western blotting and en...

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Veröffentlicht in:	Biochemical and biophysical research communications 1996-09, Vol.226 (2), p.512-516
Hauptverfasser:	Spycher, Stefan, Tabataba-Vakili, Shirin, O'Donnell, Valerie B., Palomba, Letizia, Azzi, Angelo
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehyde Reductase - genetics Aldehyde Reductase - metabolism Aldehydes - pharmacology Animals Cell Line Cell Survival - drug effects Gene Expression Regulation, Enzymologic - drug effects Muscle, Smooth - drug effects Muscle, Smooth - enzymology Rats Transcription, Genetic - drug effects
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Zusammenfassung:	Treatment of A7r5 rat vascular smooth muscle cells with 4-hydroxy-2,3-trans-nonenal (HNE) resulted in increased aldose reductase mRNA transcription. Induction was time dependent, reaching a maximum (2.3-fold) after 7 hours. An enzymatically active aldose reductase analysed by Western blotting and enzyme assays was expressed. Sorbinil, an aldose reductase inhibitor, induced a significant enhancement of HNE cytotoxicity, indicating a protective role of aldose reductase against HNE-induced A7r5 cell death. These data indicate that induction of aldose reductase by HNE may represent an important cellular defence mechanism against oxidative injury.
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1996.1386