Efficacy of a Cell-Mediated Reaction to the Purified Protein Derivative of Tuberculin in the Disposal of Mycobacterium leprae from Human Skin
The purpose of this study was to evaluate the effects of a delayed-type cell-mediated immune response to Mycobacterium tuberculosis antigen on the Mycobacterium leprae load in the skin of leprosy patients. Twelve patients with the lepromatous form of leprosy have been injected intradermally with 5 u...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-07, Vol.85 (14), p.5210-5214 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Kaplan, Gilla Sheftel, Giulia Job, C. K. Mathur, N. K. Nath, Indira Cohn, Z. A. |
| description | The purpose of this study was to evaluate the effects of a delayed-type cell-mediated immune response to Mycobacterium tuberculosis antigen on the Mycobacterium leprae load in the skin of leprosy patients. Twelve patients with the lepromatous form of leprosy have been injected intradermally with 5 units of the purified protein derivative of tuberculin (PPD). Ten individuals responded with areas of induration ranging from 12 to 21 mm in diameter, and two were unresponsive ( |
| doi_str_mv | 10.1073/pnas.85.14.5210 |
| format | Article |
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K. ; Mathur, N. K. ; Nath, Indira ; Cohn, Z. A.</creator><creatorcontrib>Kaplan, Gilla ; Sheftel, Giulia ; Job, C. K. ; Mathur, N. K. ; Nath, Indira ; Cohn, Z. A.</creatorcontrib><description>The purpose of this study was to evaluate the effects of a delayed-type cell-mediated immune response to Mycobacterium tuberculosis antigen on the Mycobacterium leprae load in the skin of leprosy patients. Twelve patients with the lepromatous form of leprosy have been injected intradermally with 5 units of the purified protein derivative of tuberculin (PPD). Ten individuals responded with areas of induration ranging from 12 to 21 mm in diameter, and two were unresponsive (<10 mm). Twenty-one days thereafter, the injected and control sites were biopsied, and the histology, number of acid-fast bacilli, nature and phenotype of the emigrant cells, and ultrastructural characteristics of the lesions were evaluated. Eight of the 10 responding patients showed reductions in the number of acid-fast bacilli by factors ranging from 5 to 10,000. Two responders and both nonresponders exhibited no discernible decline in the number of organisms. The reduction in bacillary load was correlated with an intense mononuclear cell infiltrate, the maintenance of a high CD4+ T-cell/CD8+ T-cell ratio, the formation of granulomata, and the extensive destruction of previously parasitized macrophages.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.85.14.5210</identifier><identifier>PMID: 2969108</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Antigens ; Antigens, Bacterial - immunology ; Bacterial diseases ; Biological and medical sciences ; Biopsies ; Dermis ; Giant cells ; Granuloma ; Human bacterial diseases ; Humans ; Hypersensitivity, Delayed - immunology ; Immunity, Cellular ; Infectious diseases ; Leprosy ; Leprosy - immunology ; Leprosy - pathology ; Lesions ; Macrophages ; Macrophages - immunology ; Macrophages - microbiology ; Medical sciences ; Microscopy, Electron ; Mycobacterium leprae ; Mycobacterium leprae - immunology ; Mycobacterium leprae - isolation & purification ; Mycobacterium tuberculosis ; Phagocytes - pathology ; Skin - immunology ; Skin - pathology ; T lymphocytes ; T-Lymphocytes - immunology ; T-Lymphocytes - pathology ; T-Lymphocytes, Helper-Inducer - immunology ; T-Lymphocytes, Helper-Inducer - pathology ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - pathology ; Tropical bacterial diseases ; Tropical medicine ; Tuberculin ; Tuberculin Test</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1988-07, Vol.85 (14), p.5210-5214</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-6a25dc9349f1128d1667f6eac75019be674f1e5217fba16d8cdab46a9c632caf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/85/14.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/32389$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/32389$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,778,782,801,27907,27908,58000,58233</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7187714$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2969108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaplan, Gilla</creatorcontrib><creatorcontrib>Sheftel, Giulia</creatorcontrib><creatorcontrib>Job, C. K.</creatorcontrib><creatorcontrib>Mathur, N. K.</creatorcontrib><creatorcontrib>Nath, Indira</creatorcontrib><creatorcontrib>Cohn, Z. A.</creatorcontrib><title>Efficacy of a Cell-Mediated Reaction to the Purified Protein Derivative of Tuberculin in the Disposal of Mycobacterium leprae from Human Skin</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The purpose of this study was to evaluate the effects of a delayed-type cell-mediated immune response to Mycobacterium tuberculosis antigen on the Mycobacterium leprae load in the skin of leprosy patients. Twelve patients with the lepromatous form of leprosy have been injected intradermally with 5 units of the purified protein derivative of tuberculin (PPD). Ten individuals responded with areas of induration ranging from 12 to 21 mm in diameter, and two were unresponsive (<10 mm). Twenty-one days thereafter, the injected and control sites were biopsied, and the histology, number of acid-fast bacilli, nature and phenotype of the emigrant cells, and ultrastructural characteristics of the lesions were evaluated. Eight of the 10 responding patients showed reductions in the number of acid-fast bacilli by factors ranging from 5 to 10,000. Two responders and both nonresponders exhibited no discernible decline in the number of organisms. The reduction in bacillary load was correlated with an intense mononuclear cell infiltrate, the maintenance of a high CD4+ T-cell/CD8+ T-cell ratio, the formation of granulomata, and the extensive destruction of previously parasitized macrophages.</description><subject>Antigens</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Biopsies</subject><subject>Dermis</subject><subject>Giant cells</subject><subject>Granuloma</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Hypersensitivity, Delayed - immunology</subject><subject>Immunity, Cellular</subject><subject>Infectious diseases</subject><subject>Leprosy</subject><subject>Leprosy - immunology</subject><subject>Leprosy - pathology</subject><subject>Lesions</subject><subject>Macrophages</subject><subject>Macrophages - immunology</subject><subject>Macrophages - microbiology</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Mycobacterium leprae</subject><subject>Mycobacterium leprae - immunology</subject><subject>Mycobacterium leprae - isolation & purification</subject><subject>Mycobacterium tuberculosis</subject><subject>Phagocytes - pathology</subject><subject>Skin - immunology</subject><subject>Skin - pathology</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - pathology</subject><subject>T-Lymphocytes, Helper-Inducer - immunology</subject><subject>T-Lymphocytes, Helper-Inducer - pathology</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - pathology</subject><subject>Tropical bacterial diseases</subject><subject>Tropical medicine</subject><subject>Tuberculin</subject><subject>Tuberculin Test</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-L1TAUxYMo43N0LQhKFqKrvknaNH-W8mZ0hBkcdFyX2zTBjG1Tk3TwfQi_symvPnCjEMji_M7JzT0IPadkS4mozqYR4lbWW8q2dUnJA7ShRNGCM0Ueog0hpSgkK9lj9CTGO0KIqiU5QSel4ooSuUG_Lqx1GvQee4sB70zfF9emc5BMhz8b0Mn5ESeP0zeDb-bgrMvCTfDJuBGfm-DuIbl7s9hv59YEPfdZyGcxnLs4-Qj9ol7vtW9zXrbMA-7NFMBgG_yAL-cBRvzluxufokcW-mierfcp-vr-4nZ3WVx9-vBx9-6q0IyLVHAo606riilLaSk7yrmwPA8rakJVa7hglpq8D2FboLyTuoOWcVCaV6UGW52iN4fcKfgfs4mpGVzU-e8wGj_HRsiKMqLkf0HK8iKFLDN4dgB18DEGY5spuAHCvqGkWZpqlqYaWWdLszSVHS_X6LkdTHfk12qy_nrVIWrobYBRu3jEBJVCUJaxVyu25P9R_3rn7T-Bxs59n8zPlMkXB_IuJh-OaFVWUlW_AXsjvhE</recordid><startdate>19880701</startdate><enddate>19880701</enddate><creator>Kaplan, Gilla</creator><creator>Sheftel, Giulia</creator><creator>Job, C. K.</creator><creator>Mathur, N. K.</creator><creator>Nath, Indira</creator><creator>Cohn, Z. A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19880701</creationdate><title>Efficacy of a Cell-Mediated Reaction to the Purified Protein Derivative of Tuberculin in the Disposal of Mycobacterium leprae from Human Skin</title><author>Kaplan, Gilla ; Sheftel, Giulia ; Job, C. K. ; Mathur, N. K. ; Nath, Indira ; Cohn, Z. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-6a25dc9349f1128d1667f6eac75019be674f1e5217fba16d8cdab46a9c632caf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Antigens</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Biopsies</topic><topic>Dermis</topic><topic>Giant cells</topic><topic>Granuloma</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Hypersensitivity, Delayed - immunology</topic><topic>Immunity, Cellular</topic><topic>Infectious diseases</topic><topic>Leprosy</topic><topic>Leprosy - immunology</topic><topic>Leprosy - pathology</topic><topic>Lesions</topic><topic>Macrophages</topic><topic>Macrophages - immunology</topic><topic>Macrophages - microbiology</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Mycobacterium leprae</topic><topic>Mycobacterium leprae - immunology</topic><topic>Mycobacterium leprae - isolation & purification</topic><topic>Mycobacterium tuberculosis</topic><topic>Phagocytes - pathology</topic><topic>Skin - immunology</topic><topic>Skin - pathology</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - pathology</topic><topic>T-Lymphocytes, Helper-Inducer - immunology</topic><topic>T-Lymphocytes, Helper-Inducer - pathology</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - pathology</topic><topic>Tropical bacterial diseases</topic><topic>Tropical medicine</topic><topic>Tuberculin</topic><topic>Tuberculin Test</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaplan, Gilla</creatorcontrib><creatorcontrib>Sheftel, Giulia</creatorcontrib><creatorcontrib>Job, C. K.</creatorcontrib><creatorcontrib>Mathur, N. K.</creatorcontrib><creatorcontrib>Nath, Indira</creatorcontrib><creatorcontrib>Cohn, Z. A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaplan, Gilla</au><au>Sheftel, Giulia</au><au>Job, C. K.</au><au>Mathur, N. K.</au><au>Nath, Indira</au><au>Cohn, Z. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of a Cell-Mediated Reaction to the Purified Protein Derivative of Tuberculin in the Disposal of Mycobacterium leprae from Human Skin</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-07-01</date><risdate>1988</risdate><volume>85</volume><issue>14</issue><spage>5210</spage><epage>5214</epage><pages>5210-5214</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The purpose of this study was to evaluate the effects of a delayed-type cell-mediated immune response to Mycobacterium tuberculosis antigen on the Mycobacterium leprae load in the skin of leprosy patients. Twelve patients with the lepromatous form of leprosy have been injected intradermally with 5 units of the purified protein derivative of tuberculin (PPD). Ten individuals responded with areas of induration ranging from 12 to 21 mm in diameter, and two were unresponsive (<10 mm). Twenty-one days thereafter, the injected and control sites were biopsied, and the histology, number of acid-fast bacilli, nature and phenotype of the emigrant cells, and ultrastructural characteristics of the lesions were evaluated. Eight of the 10 responding patients showed reductions in the number of acid-fast bacilli by factors ranging from 5 to 10,000. Two responders and both nonresponders exhibited no discernible decline in the number of organisms. The reduction in bacillary load was correlated with an intense mononuclear cell infiltrate, the maintenance of a high CD4+ T-cell/CD8+ T-cell ratio, the formation of granulomata, and the extensive destruction of previously parasitized macrophages.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2969108</pmid><doi>10.1073/pnas.85.14.5210</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1988-07, Vol.85 (14), p.5210-5214 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78314098 |
| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Antigens Antigens, Bacterial - immunology Bacterial diseases Biological and medical sciences Biopsies Dermis Giant cells Granuloma Human bacterial diseases Humans Hypersensitivity, Delayed - immunology Immunity, Cellular Infectious diseases Leprosy Leprosy - immunology Leprosy - pathology Lesions Macrophages Macrophages - immunology Macrophages - microbiology Medical sciences Microscopy, Electron Mycobacterium leprae Mycobacterium leprae - immunology Mycobacterium leprae - isolation & purification Mycobacterium tuberculosis Phagocytes - pathology Skin - immunology Skin - pathology T lymphocytes T-Lymphocytes - immunology T-Lymphocytes - pathology T-Lymphocytes, Helper-Inducer - immunology T-Lymphocytes, Helper-Inducer - pathology T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - pathology Tropical bacterial diseases Tropical medicine Tuberculin Tuberculin Test |
| title | Efficacy of a Cell-Mediated Reaction to the Purified Protein Derivative of Tuberculin in the Disposal of Mycobacterium leprae from Human Skin |
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