Cytoplasmic Poly(ADP‐Ribose) Polymerase Associated with Free Messenger Ribonucleoprotein Particles in Rat Brain

: Poly(ADP‐ribose) polymerase associated with free cytoplasmic messenger ribonucleoprotein particles (free mRNP particles) carrying messenger RNA has been characterized in rat brain. There were first‐order kinetics for NAD with an apparent Km for NAD of 90.5 ± 0.70 μM and Vmax of 19.7 ± 2.8 pmol ADP...

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Veröffentlicht in:	Journal of neurochemistry 1988-08, Vol.51 (2), p.561-565
Hauptverfasser:	Chypre, Camille, Maniez, Christine, Mandel, Paul
Format:	Artikel
Sprache:	eng
Schlagworte:	ADP‐ribosylation Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Brain Brain - enzymology Brain - ultrastructure Cations, Divalent - pharmacology Free messenger ribonucleoprotein particle Fundamental and applied biological sciences. Psychology Kinetics NAD - metabolism Nucleic acids Poly(ADP-ribose) Polymerases - metabolism Poly(ADP‐ribose) polymerase Rat Rats Ribonucleoproteins - metabolism Rna, ribonucleoproteins
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Zusammenfassung:	: Poly(ADP‐ribose) polymerase associated with free cytoplasmic messenger ribonucleoprotein particles (free mRNP particles) carrying messenger RNA has been characterized in rat brain. There were first‐order kinetics for NAD with an apparent Km for NAD of 90.5 ± 0.70 μM and Vmax of 19.7 ± 2.8 pmol ADP‐ribose incorporated min‐1 mg protein‐1. Five poly(ADP‐ribose) protein acceptors were identified in the Mr 37,000–120,000 range. It is hypothesized that ADP‐ribosylation of specific free mRNP proteins might play a role in the derepression and translation of the silent mRNAs of free mRNP particles.
ISSN:	0022-3042 1471-4159
DOI:	10.1111/j.1471-4159.1988.tb01075.x