A quantitative evaluation of promoter activity by microinjection of chloramphenicol acetyltransferase hybrid genes into tissue culture cells

The combination of DNA microinjection into tissue culture cells and the chloramphenicol acetyltransferase enzyme assay has been used to determine quantitatively the transcriptional activity of viral or eukaryotic promoters. As shown for a wide range of DNA concentrations and time periods between 2 a...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 1988-04, Vol.170 (1), p.243-247
Hauptverfasser:	Buschhausen-Denker, G., Arnold, H.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyltransferases - analysis Acetyltransferases - genetics Animals Biological and medical sciences Biotechnology Cells, Cultured chloramphenicol acetyltransferase Chloramphenicol O-Acetyltransferase Fibroblasts - enzymology Fundamental and applied biological sciences. Psychology gene expression and regulation Genetic engineering Genetic technics Haplorhini Hybrid Cells Kidney - enzymology Methods. Procedures. Technologies microinjection Microinjections Miscellaneous Promoter Regions, Genetic recombinant technology Synthetic digonucleotides and genes. Sequencing Transcription, Genetic transient expression
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The combination of DNA microinjection into tissue culture cells and the chloramphenicol acetyltransferase enzyme assay has been used to determine quantitatively the transcriptional activity of viral or eukaryotic promoters. As shown for a wide range of DNA concentrations and time periods between 2 and 16 h the assay allows the reliable and reproducible determination of promoter activities in defined cellular backgrounds. Only 50 cells are necessary for the test.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(88)90114-5