Monoclonal antibodies directed against the calcium binding protein parvalbumin

We have produced twelve monoclonal antibodies (McAB) against carp-II parvalbumin. Three of them, designated 235, 239, 267 recognize determinants conserved in fish, chicken, mouse, rat, monkey and human parvalbumin. We show their use in the qualitative detection of parvalbumin (PV) by immunohistochem...

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Veröffentlicht in:	Cell calcium (Edinburgh) 1988-04, Vol.9 (2), p.81-86
Hauptverfasser:	Celio, Marco R., Baier, W., Schärer, L., De Viragh, P.A., Gerday, Ch
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - immunology Binding and carrier proteins Biological and medical sciences Calcium-Binding Proteins - metabolism Carps Cyprinus carpio Fundamental and applied biological sciences. Psychology Muscle Proteins - immunology Parvalbumins - immunology Parvalbumins - metabolism Parvalbumins - physiology Proteins
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Beschreibung
Zusammenfassung:	We have produced twelve monoclonal antibodies (McAB) against carp-II parvalbumin. Three of them, designated 235, 239, 267 recognize determinants conserved in fish, chicken, mouse, rat, monkey and human parvalbumin. We show their use in the qualitative detection of parvalbumin (PV) by immunohistochemistry, in the quantitation of parvalbumin by radioimmunoassay and in the detection of parvalbumin on immunoblots.
ISSN:	0143-4160 1532-1991
DOI:	10.1016/0143-4160(88)90027-9