EVALUATION OF AN ENZYME IMMUNOASSAY TEST FOR THE DIAGNOSIS OF CHLAMYDIA PSITTACI INFECTION IN FREE-RANGING KOALAS (PHASCOLARCTOS CINEREUS) IN SOUTHEASTERN QUEENSLAND, AUSTRALIA

The IDEIA Chlamydia Test, a commercially available antigen-capture enzyme-linked immunosorbent assay (ELISA) test, based on a monoclonal antibody for the detection of chlamydia in clinical specimens, was evaluated in a population of 65 free-ranging koalas in southeastern Queensland determined to be...

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Veröffentlicht in:	Journal of wildlife diseases 1988-04, Vol.24 (2), p.259-263
Hauptverfasser:	Weigler, Benjamin J., Baldock, F. Christian, Girjes, Adeeb A., Carrick, Frank N., Lavin, Martin F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Wild Chlamydia Infections - diagnosis Chlamydia Infections - veterinary Chlamydia psittaci Chlamydophila psittaci Conjunctival Diseases - diagnosis Conjunctival Diseases - etiology Conjunctival Diseases - veterinary ELISA screening test Enzyme-Linked Immunosorbent Assay - veterinary enzyme-linked immunosorbent assay test evaluation Evaluation Studies as Topic koala Marsupialia - microbiology Mass Screening Phascolarctos cinereus Queensland Urologic Diseases - diagnosis Urologic Diseases - etiology Urologic Diseases - veterinary
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Beschreibung
Zusammenfassung:	The IDEIA Chlamydia Test, a commercially available antigen-capture enzyme-linked immunosorbent assay (ELISA) test, based on a monoclonal antibody for the detection of chlamydia in clinical specimens, was evaluated in a population of 65 free-ranging koalas in southeastern Queensland determined to be infected with Chlamydia psittaci. Compared to isolation of the organism in tissue culture, the sensitivity of the IDEIA test ranged from 3 to 11%, and the specificity from 90 to 97%. The results indicated that the IDEIA test is unsuitable for use as a diagnostic screening test for C. psittaci in free-ranging koalas.
ISSN:	0090-3558 1943-3700
DOI:	10.7589/0090-3558-24.2.259