Identification of a murine CD4 + T-lymphocyte response site in hepatitis C virus core protein
The T cell response to a recombinant HCV truncated core protein (cp1-10) was measured in a proliferation assay. Based on a 10-fold greater response to this truncated core protein than to its shorter form (cp1-8), a predominant epitope was mapped to the carboxyl quarter of this sequence. This epitope...
Gespeichert in:
| Veröffentlicht in: | Molecular immunology 1996-05, Vol.33 (7), p.703-709 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 709 |
|---|---|
| container_issue | 7 |
| container_start_page | 703 |
| container_title | Molecular immunology |
| container_volume | 33 |
| creator | Chen, Ziping Berkower, Ira Ching, Wei-Mei Wang, R.Yuan-Hu Alter, Harvey J. Shih, J.Wai-kuo |
| description | The T cell response to a recombinant HCV truncated core protein (cp1-10) was measured in a proliferation assay. Based on a 10-fold greater response to this truncated core protein than to its shorter form (cp1-8), a predominant epitope was mapped to the carboxyl quarter of this sequence. This epitope was further mapped to a synthetic peptide corresponding to amino acids 121–140 of the core protein. The peptide was antigenic for T cells of all three H-2 types tested, H-2 r, b and d, and the proliferating T cells were CD4
+. Besides inducing specific proliferation
in vitro, peptide aa121–140 can prime helper T cells
in vivo. When boosted with core protein, mice primed with peptide produced 64-fold higher antibody titer than without priming in 1 week. The identification of a broadly immunogenic T cell helper epitope on core protein may be important for vaccine design against HCV. |
| doi_str_mv | 10.1016/0161-5890(96)00010-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78229872</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0161589096000107</els_id><sourcerecordid>78229872</sourcerecordid><originalsourceid>FETCH-LOGICAL-c357t-80df49934fbc03c82f2bcf76b2a3a040687230167a91873c3f6964d2c1048f723</originalsourceid><addsrcrecordid>eNp9kF9LwzAUxYMoc06_gUKeRJFqknZp8iLI_DcY-DIfJbTpDYu0TU3awb69mRt79CGEyznnXs4PoUtK7imh_CE-mkyFJDeS3xJCKEnyIzSmImeJpBk7RuOD5RSdhfAdTZzw6QiNRM4JE2yMvuYVtL01Vhe9dS12Bhe4GbxtAc-eM3yHl0m9abqV05sesIfQuTYADjZOtsUr6GKwtwHP8Nr6IWDtPODOux5se45OTFEHuNj_E_T5-rKcvSeLj7f57GmR6HSa94kglcmkTDNTapJqwQwrtcl5yYq0IBnhsVIau-SFjO1SnRoueVYxTUkmTNQm6Hq3N979GSD0qrFBQ10XLbghqFwwJsWfMdsZtXcheDCq87Yp_EZRorZU1RaZ2iJTMg5bqiqPsav9_qFsoDqE9hij_rjTIZZcW_AqaAuthsp60L2qnP3_wC_2IYTz</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78229872</pqid></control><display><type>article</type><title>Identification of a murine CD4 + T-lymphocyte response site in hepatitis C virus core protein</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Chen, Ziping ; Berkower, Ira ; Ching, Wei-Mei ; Wang, R.Yuan-Hu ; Alter, Harvey J. ; Shih, J.Wai-kuo</creator><creatorcontrib>Chen, Ziping ; Berkower, Ira ; Ching, Wei-Mei ; Wang, R.Yuan-Hu ; Alter, Harvey J. ; Shih, J.Wai-kuo</creatorcontrib><description>The T cell response to a recombinant HCV truncated core protein (cp1-10) was measured in a proliferation assay. Based on a 10-fold greater response to this truncated core protein than to its shorter form (cp1-8), a predominant epitope was mapped to the carboxyl quarter of this sequence. This epitope was further mapped to a synthetic peptide corresponding to amino acids 121–140 of the core protein. The peptide was antigenic for T cells of all three H-2 types tested, H-2 r, b and d, and the proliferating T cells were CD4
+. Besides inducing specific proliferation
in vitro, peptide aa121–140 can prime helper T cells
in vivo. When boosted with core protein, mice primed with peptide produced 64-fold higher antibody titer than without priming in 1 week. The identification of a broadly immunogenic T cell helper epitope on core protein may be important for vaccine design against HCV.</description><identifier>ISSN: 0161-5890</identifier><identifier>EISSN: 1872-9142</identifier><identifier>DOI: 10.1016/0161-5890(96)00010-7</identifier><identifier>PMID: 8760282</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; CD4-Positive T-Lymphocytes - drug effects ; CD4-Positive T-Lymphocytes - immunology ; epitope mapping ; H-2 congenic mice ; HCV ; Hepacivirus - immunology ; immune responses ; Immunization ; Lymphocyte Activation - drug effects ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Peptide Fragments - immunology ; T helper epitope ; T-Lymphocyte Subsets - drug effects ; T-Lymphocyte Subsets - immunology ; T-Lymphocytes, Helper-Inducer - drug effects ; T-Lymphocytes, Helper-Inducer - immunology ; truncated recombinant core proteins ; Viral Core Proteins - immunology ; Viral Core Proteins - pharmacology</subject><ispartof>Molecular immunology, 1996-05, Vol.33 (7), p.703-709</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-80df49934fbc03c82f2bcf76b2a3a040687230167a91873c3f6964d2c1048f723</citedby><cites>FETCH-LOGICAL-c357t-80df49934fbc03c82f2bcf76b2a3a040687230167a91873c3f6964d2c1048f723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0161589096000107$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8760282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Ziping</creatorcontrib><creatorcontrib>Berkower, Ira</creatorcontrib><creatorcontrib>Ching, Wei-Mei</creatorcontrib><creatorcontrib>Wang, R.Yuan-Hu</creatorcontrib><creatorcontrib>Alter, Harvey J.</creatorcontrib><creatorcontrib>Shih, J.Wai-kuo</creatorcontrib><title>Identification of a murine CD4 + T-lymphocyte response site in hepatitis C virus core protein</title><title>Molecular immunology</title><addtitle>Mol Immunol</addtitle><description>The T cell response to a recombinant HCV truncated core protein (cp1-10) was measured in a proliferation assay. Based on a 10-fold greater response to this truncated core protein than to its shorter form (cp1-8), a predominant epitope was mapped to the carboxyl quarter of this sequence. This epitope was further mapped to a synthetic peptide corresponding to amino acids 121–140 of the core protein. The peptide was antigenic for T cells of all three H-2 types tested, H-2 r, b and d, and the proliferating T cells were CD4
+. Besides inducing specific proliferation
in vitro, peptide aa121–140 can prime helper T cells
in vivo. When boosted with core protein, mice primed with peptide produced 64-fold higher antibody titer than without priming in 1 week. The identification of a broadly immunogenic T cell helper epitope on core protein may be important for vaccine design against HCV.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>CD4-Positive T-Lymphocytes - drug effects</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>epitope mapping</subject><subject>H-2 congenic mice</subject><subject>HCV</subject><subject>Hepacivirus - immunology</subject><subject>immune responses</subject><subject>Immunization</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Sequence Data</subject><subject>Peptide Fragments - immunology</subject><subject>T helper epitope</subject><subject>T-Lymphocyte Subsets - drug effects</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocytes, Helper-Inducer - drug effects</subject><subject>T-Lymphocytes, Helper-Inducer - immunology</subject><subject>truncated recombinant core proteins</subject><subject>Viral Core Proteins - immunology</subject><subject>Viral Core Proteins - pharmacology</subject><issn>0161-5890</issn><issn>1872-9142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9LwzAUxYMoc06_gUKeRJFqknZp8iLI_DcY-DIfJbTpDYu0TU3awb69mRt79CGEyznnXs4PoUtK7imh_CE-mkyFJDeS3xJCKEnyIzSmImeJpBk7RuOD5RSdhfAdTZzw6QiNRM4JE2yMvuYVtL01Vhe9dS12Bhe4GbxtAc-eM3yHl0m9abqV05sesIfQuTYADjZOtsUr6GKwtwHP8Nr6IWDtPODOux5se45OTFEHuNj_E_T5-rKcvSeLj7f57GmR6HSa94kglcmkTDNTapJqwQwrtcl5yYq0IBnhsVIau-SFjO1SnRoueVYxTUkmTNQm6Hq3N979GSD0qrFBQ10XLbghqFwwJsWfMdsZtXcheDCq87Yp_EZRorZU1RaZ2iJTMg5bqiqPsav9_qFsoDqE9hij_rjTIZZcW_AqaAuthsp60L2qnP3_wC_2IYTz</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Chen, Ziping</creator><creator>Berkower, Ira</creator><creator>Ching, Wei-Mei</creator><creator>Wang, R.Yuan-Hu</creator><creator>Alter, Harvey J.</creator><creator>Shih, J.Wai-kuo</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960501</creationdate><title>Identification of a murine CD4 + T-lymphocyte response site in hepatitis C virus core protein</title><author>Chen, Ziping ; Berkower, Ira ; Ching, Wei-Mei ; Wang, R.Yuan-Hu ; Alter, Harvey J. ; Shih, J.Wai-kuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-80df49934fbc03c82f2bcf76b2a3a040687230167a91873c3f6964d2c1048f723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>CD4-Positive T-Lymphocytes - drug effects</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>epitope mapping</topic><topic>H-2 congenic mice</topic><topic>HCV</topic><topic>Hepacivirus - immunology</topic><topic>immune responses</topic><topic>Immunization</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Sequence Data</topic><topic>Peptide Fragments - immunology</topic><topic>T helper epitope</topic><topic>T-Lymphocyte Subsets - drug effects</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocytes, Helper-Inducer - drug effects</topic><topic>T-Lymphocytes, Helper-Inducer - immunology</topic><topic>truncated recombinant core proteins</topic><topic>Viral Core Proteins - immunology</topic><topic>Viral Core Proteins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Ziping</creatorcontrib><creatorcontrib>Berkower, Ira</creatorcontrib><creatorcontrib>Ching, Wei-Mei</creatorcontrib><creatorcontrib>Wang, R.Yuan-Hu</creatorcontrib><creatorcontrib>Alter, Harvey J.</creatorcontrib><creatorcontrib>Shih, J.Wai-kuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Ziping</au><au>Berkower, Ira</au><au>Ching, Wei-Mei</au><au>Wang, R.Yuan-Hu</au><au>Alter, Harvey J.</au><au>Shih, J.Wai-kuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a murine CD4 + T-lymphocyte response site in hepatitis C virus core protein</atitle><jtitle>Molecular immunology</jtitle><addtitle>Mol Immunol</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>33</volume><issue>7</issue><spage>703</spage><epage>709</epage><pages>703-709</pages><issn>0161-5890</issn><eissn>1872-9142</eissn><abstract>The T cell response to a recombinant HCV truncated core protein (cp1-10) was measured in a proliferation assay. Based on a 10-fold greater response to this truncated core protein than to its shorter form (cp1-8), a predominant epitope was mapped to the carboxyl quarter of this sequence. This epitope was further mapped to a synthetic peptide corresponding to amino acids 121–140 of the core protein. The peptide was antigenic for T cells of all three H-2 types tested, H-2 r, b and d, and the proliferating T cells were CD4
+. Besides inducing specific proliferation
in vitro, peptide aa121–140 can prime helper T cells
in vivo. When boosted with core protein, mice primed with peptide produced 64-fold higher antibody titer than without priming in 1 week. The identification of a broadly immunogenic T cell helper epitope on core protein may be important for vaccine design against HCV.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>8760282</pmid><doi>10.1016/0161-5890(96)00010-7</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0161-5890 |
| ispartof | Molecular immunology, 1996-05, Vol.33 (7), p.703-709 |
| issn | 0161-5890 1872-9142 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78229872 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals CD4-Positive T-Lymphocytes - drug effects CD4-Positive T-Lymphocytes - immunology epitope mapping H-2 congenic mice HCV Hepacivirus - immunology immune responses Immunization Lymphocyte Activation - drug effects Mice Mice, Inbred C57BL Molecular Sequence Data Peptide Fragments - immunology T helper epitope T-Lymphocyte Subsets - drug effects T-Lymphocyte Subsets - immunology T-Lymphocytes, Helper-Inducer - drug effects T-Lymphocytes, Helper-Inducer - immunology truncated recombinant core proteins Viral Core Proteins - immunology Viral Core Proteins - pharmacology |
| title | Identification of a murine CD4 + T-lymphocyte response site in hepatitis C virus core protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T01%3A15%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20a%20murine%20CD4%20+%20T-lymphocyte%20response%20site%20in%20hepatitis%20C%20virus%20core%20protein&rft.jtitle=Molecular%20immunology&rft.au=Chen,%20Ziping&rft.date=1996-05-01&rft.volume=33&rft.issue=7&rft.spage=703&rft.epage=709&rft.pages=703-709&rft.issn=0161-5890&rft.eissn=1872-9142&rft_id=info:doi/10.1016/0161-5890(96)00010-7&rft_dat=%3Cproquest_cross%3E78229872%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78229872&rft_id=info:pmid/8760282&rft_els_id=0161589096000107&rfr_iscdi=true |