Characterization of Borrelia sp. isolated from Ixodes tanuki, I. turdus, and I. columnae in Japan by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons

Borrelia isolated from various sources in Japan, including rare species of ixodid ticks, Ixodes tanuki, I. turdus, and I. columnae, were characterized by restriction fragment length polymorphism analysis and sequencing analysis of the 5S-23S rRNA intergenic spacer amplicon. Borrelia sp. isolated fro...

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Veröffentlicht in:	FEMS microbiology letters 1996-08, Vol.142 (1), p.77-83
Hauptverfasser:	Masuzawa, T, Komikado, T, Iwaki, A, Suzuki, H, Kaneda, K, Yanagihara, Y
Format:	Artikel
Sprache:	eng
Schlagworte:	animal health animal parasites and pests Animals arthropod pests Bacteriology Base Sequence Biological and medical sciences Borrelia Borrelia - genetics Borrelia - isolation & purification Borrelia burgdorferi Borrelia burgdorferi Group - genetics Borrelia burgdorferi Group - isolation & purification DNA Primers - genetics Epidemiology Fundamental and applied biological sciences. Psychology Gene polymorphism Humans Intergenic spacer Ixodes Ixodes - microbiology Japan Lyme disease Microbiology Molecular Sequence Data Polymerase chain reaction Polymorphism Polymorphism, Restriction Fragment Length Rare species Restriction fragment length polymorphism RNA, Bacterial - genetics RNA, Ribosomal, 23S - genetics RNA, Ribosomal, 5S - genetics rRNA rRNA 23S rRNA 5S Sequence analysis Species Specificity Tick Ticks
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Zusammenfassung:	Borrelia isolated from various sources in Japan, including rare species of ixodid ticks, Ixodes tanuki, I. turdus, and I. columnae, were characterized by restriction fragment length polymorphism analysis and sequencing analysis of the 5S-23S rRNA intergenic spacer amplicon. Borrelia sp. isolated from I. tanuki, I. turdus and I. columnae generated restriction fragment length polymorphism patterns different from those of known B. burgdorferi sensu lato isolates previously reported. Furthermore, some B. afzelii and B. garinii isolated in Japan showed unique RFLP patterns which were not observed among European B. afzelii and B. garinii.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1996.tb08411.x