Opioid regulation of intracellular free calcium in cultured mouse dorsal root ganglion neurons

Opioid agonists induced an increase in the intracellular free calcium concentration ([Ca2+]i) or an inhibition of K+ (25 mM)‐stimulated increase in [Ca2+]i in different subsets of mouse dorsal root ganglion (DRG) neurons. The total neuronal population was grouped into three classes according to soma...

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Veröffentlicht in:Journal of neuroscience research 1996-05, Vol.44 (4), p.338-343
Hauptverfasser: Tang, T., Stevens, B.A., Cox, B.M.
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Sprache:eng
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Zusammenfassung:Opioid agonists induced an increase in the intracellular free calcium concentration ([Ca2+]i) or an inhibition of K+ (25 mM)‐stimulated increase in [Ca2+]i in different subsets of mouse dorsal root ganglion (DRG) neurons. The total neuronal population was grouped into three classes according to somatic diameter and defined as small (25 μm) neurons. Substance P‐like immunoreactivity was detected mainly in the small and intermediate neurons. The δ, κ, and μ opioid receptor agonists [D‐Ser2, Leu5]enkephalin‐Thr (DSLET), U69593, and [D‐Ala2, MePhe4, Gly‐ol5]enkephalin (DAMGO) each induced a transient increase in [Ca2+]i in a small fraction ( U69593 > DAMGO. The opioid‐induced increase in [Ca2+]i was observed mainly in large neurons, with a low incidence in small and intermediate neurons. Opioid agonists also caused inhibition of K+‐stimulated increases in [Ca2+]i, which were blocked by naloxone (1 μM). Inhibition of the K+‐stimulated increase by 1 μM DSLET or U69593 was greater in small and intermediate neurons than in large neurons. © 1996 Wiley‐Liss, Inc.
ISSN:0360-4012
1097-4547
DOI:10.1002/(SICI)1097-4547(19960515)44:4<338::AID-JNR4>3.0.CO;2-D