An improved method for rapid analysis of the fatty acids of glycerolipids
An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature...
Gespeichert in:
| Veröffentlicht in: | Lipids 1996-05, Vol.31 (5), p.535-539 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 539 |
|---|---|
| container_issue | 5 |
| container_start_page | 535 |
| container_title | Lipids |
| container_volume | 31 |
| creator | Ichihara, Ken’ichi Shibahara, Akira Yamamoto, Kohei Nakayama, Takao |
| description | An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP‐2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils. |
| doi_str_mv | 10.1007/BF02522648 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78216457</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78216457</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3895-2fdf79ba33ba1a3a21db8b3180517e2f46e16d7de6141f6d997b6acc99b8e8c23</originalsourceid><addsrcrecordid>eNp9kDtLA0EURgdRYow29sJUFsLqvB-lRqOBgBZaL7PzMCO72TizUfbfu5qgndXl3u_wcTkAnGJ0iRGSVzczRDghgqk9MMacq0JTJPfBGCHCCkYQPgRHOb8NK2aaj8BISSIFk2Mwv17B2KxT--EdbHy3bB0MbYLJrKODZmXqPscM2wC7pYfBdF0PjY3u5_Ra99anto4Dm4_BQTB19ie7OQEvs7vn6UOxeLyfT68XhaVK84IEF6SuDKWVwYYagl2lKooV4lh6EpjwWDjpvMAMB-G0lpUw1mpdKa8soRNwvu0dnn7f-NyVTczW17VZ-XaTS6kIFozLAbzYgja1OScfynWKjUl9iVH57a388zbAZ7vWTdV494vuRA052uafsfb9P03lYv50izjl9AtRlXXa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78216457</pqid></control><display><type>article</type><title>An improved method for rapid analysis of the fatty acids of glycerolipids</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Springer Nature - Complete Springer Journals</source><creator>Ichihara, Ken’ichi ; Shibahara, Akira ; Yamamoto, Kohei ; Nakayama, Takao</creator><creatorcontrib>Ichihara, Ken’ichi ; Shibahara, Akira ; Yamamoto, Kohei ; Nakayama, Takao</creatorcontrib><description>An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP‐2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils.</description><identifier>ISSN: 0024-4201</identifier><identifier>EISSN: 1558-9307</identifier><identifier>DOI: 10.1007/BF02522648</identifier><identifier>PMID: 8727647</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer‐Verlag</publisher><subject>Cholesterol Esters - analysis ; Chromatography, Gas - methods ; Chromatography, Thin Layer - methods ; Esters ; Fatty Acids - analysis ; Galactolipids ; Glycerides - chemistry ; Glycolipids - analysis ; Hydroxides ; Oleic Acids - analysis ; Potassium Compounds ; Sensitivity and Specificity ; Solvents ; Triglycerides - chemistry</subject><ispartof>Lipids, 1996-05, Vol.31 (5), p.535-539</ispartof><rights>1996 American Oil Chemists' Society (AOCS)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3895-2fdf79ba33ba1a3a21db8b3180517e2f46e16d7de6141f6d997b6acc99b8e8c23</citedby><cites>FETCH-LOGICAL-c3895-2fdf79ba33ba1a3a21db8b3180517e2f46e16d7de6141f6d997b6acc99b8e8c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1007%2FBF02522648$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1007%2FBF02522648$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8727647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ichihara, Ken’ichi</creatorcontrib><creatorcontrib>Shibahara, Akira</creatorcontrib><creatorcontrib>Yamamoto, Kohei</creatorcontrib><creatorcontrib>Nakayama, Takao</creatorcontrib><title>An improved method for rapid analysis of the fatty acids of glycerolipids</title><title>Lipids</title><addtitle>Lipids</addtitle><description>An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP‐2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils.</description><subject>Cholesterol Esters - analysis</subject><subject>Chromatography, Gas - methods</subject><subject>Chromatography, Thin Layer - methods</subject><subject>Esters</subject><subject>Fatty Acids - analysis</subject><subject>Galactolipids</subject><subject>Glycerides - chemistry</subject><subject>Glycolipids - analysis</subject><subject>Hydroxides</subject><subject>Oleic Acids - analysis</subject><subject>Potassium Compounds</subject><subject>Sensitivity and Specificity</subject><subject>Solvents</subject><subject>Triglycerides - chemistry</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtLA0EURgdRYow29sJUFsLqvB-lRqOBgBZaL7PzMCO72TizUfbfu5qgndXl3u_wcTkAnGJ0iRGSVzczRDghgqk9MMacq0JTJPfBGCHCCkYQPgRHOb8NK2aaj8BISSIFk2Mwv17B2KxT--EdbHy3bB0MbYLJrKODZmXqPscM2wC7pYfBdF0PjY3u5_Ra99anto4Dm4_BQTB19ie7OQEvs7vn6UOxeLyfT68XhaVK84IEF6SuDKWVwYYagl2lKooV4lh6EpjwWDjpvMAMB-G0lpUw1mpdKa8soRNwvu0dnn7f-NyVTczW17VZ-XaTS6kIFozLAbzYgja1OScfynWKjUl9iVH57a388zbAZ7vWTdV494vuRA052uafsfb9P03lYv50izjl9AtRlXXa</recordid><startdate>199605</startdate><enddate>199605</enddate><creator>Ichihara, Ken’ichi</creator><creator>Shibahara, Akira</creator><creator>Yamamoto, Kohei</creator><creator>Nakayama, Takao</creator><general>Springer‐Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199605</creationdate><title>An improved method for rapid analysis of the fatty acids of glycerolipids</title><author>Ichihara, Ken’ichi ; Shibahara, Akira ; Yamamoto, Kohei ; Nakayama, Takao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3895-2fdf79ba33ba1a3a21db8b3180517e2f46e16d7de6141f6d997b6acc99b8e8c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Cholesterol Esters - analysis</topic><topic>Chromatography, Gas - methods</topic><topic>Chromatography, Thin Layer - methods</topic><topic>Esters</topic><topic>Fatty Acids - analysis</topic><topic>Galactolipids</topic><topic>Glycerides - chemistry</topic><topic>Glycolipids - analysis</topic><topic>Hydroxides</topic><topic>Oleic Acids - analysis</topic><topic>Potassium Compounds</topic><topic>Sensitivity and Specificity</topic><topic>Solvents</topic><topic>Triglycerides - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichihara, Ken’ichi</creatorcontrib><creatorcontrib>Shibahara, Akira</creatorcontrib><creatorcontrib>Yamamoto, Kohei</creatorcontrib><creatorcontrib>Nakayama, Takao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichihara, Ken’ichi</au><au>Shibahara, Akira</au><au>Yamamoto, Kohei</au><au>Nakayama, Takao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved method for rapid analysis of the fatty acids of glycerolipids</atitle><jtitle>Lipids</jtitle><addtitle>Lipids</addtitle><date>1996-05</date><risdate>1996</risdate><volume>31</volume><issue>5</issue><spage>535</spage><epage>539</epage><pages>535-539</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><abstract>An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP‐2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer‐Verlag</pub><pmid>8727647</pmid><doi>10.1007/BF02522648</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-4201 |
| ispartof | Lipids, 1996-05, Vol.31 (5), p.535-539 |
| issn | 0024-4201 1558-9307 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78216457 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Springer Nature - Complete Springer Journals |
| subjects | Cholesterol Esters - analysis Chromatography, Gas - methods Chromatography, Thin Layer - methods Esters Fatty Acids - analysis Galactolipids Glycerides - chemistry Glycolipids - analysis Hydroxides Oleic Acids - analysis Potassium Compounds Sensitivity and Specificity Solvents Triglycerides - chemistry |
| title | An improved method for rapid analysis of the fatty acids of glycerolipids |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T08%3A09%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20improved%20method%20for%20rapid%20analysis%20of%20the%20fatty%20acids%20of%20glycerolipids&rft.jtitle=Lipids&rft.au=Ichihara,%20Ken%E2%80%99ichi&rft.date=1996-05&rft.volume=31&rft.issue=5&rft.spage=535&rft.epage=539&rft.pages=535-539&rft.issn=0024-4201&rft.eissn=1558-9307&rft_id=info:doi/10.1007/BF02522648&rft_dat=%3Cproquest_cross%3E78216457%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78216457&rft_id=info:pmid/8727647&rfr_iscdi=true |