An improved method for rapid analysis of the fatty acids of glycerolipids

An improved method for rapid analysis of the fatty acids of glycerolipids

An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature...

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Veröffentlicht in:Lipids 1996-05, Vol.31 (5), p.535-539
Hauptverfasser: Ichihara, Ken’ichi, Shibahara, Akira, Yamamoto, Kohei, Nakayama, Takao
Format: Artikel
Sprache:eng
Schlagworte:
Cholesterol Esters - analysis
Chromatography, Gas - methods
Chromatography, Thin Layer - methods
Esters
Fatty Acids - analysis
Galactolipids
Glycerides - chemistry
Glycolipids - analysis
Hydroxides
Oleic Acids - analysis
Potassium Compounds
Sensitivity and Specificity
Solvents
Triglycerides - chemistry
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Zusammenfassung:An improved rapid procedure to determine the fatty acid composition of glycerolipids is described. The procedure includes KOH‐catalyzed transesterification and high‐speed gas chromatography. Glycerolipids (20–40 mg) were mixed with 2 mL of hexane and 0.2 mL of 2 M methanolic KOH at room temperature for 1–2 min. The fatty acid methyl esters in the hexane layer were analyzed by gas chromatography on 10% SP‐2340 at 240°C. Methyl linolenate and docosahexaenoate eluted within 2 and 5 min, respectively. Analysis was thus completed with 5 min for common vegetable oils and 8 min for fish oils.
ISSN:0024-4201
1558-9307
DOI:10.1007/BF02522648