45-KDa GTP-binding Protein from Rat Olfactory Epithelium: Purification, Characterization and Localization

The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with α-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45...

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Veröffentlicht in:	Chemical senses 1996-04, Vol.21 (2), p.181-188
Hauptverfasser:	Novoselov, V.I., Peshenko, I.V., Evdokimov, V.A., Nikolaev, J.V., Matveeva, E.A., Fesenko, E.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Amino Acid Sequence Animals Antibodies Epithelium - chemistry GTP-Binding Proteins - chemistry GTP-Binding Proteins - immunology GTP-Binding Proteins - isolation & purification Guanosine Triphosphate - chemistry Immunoblotting Male Molecular Sequence Data Molecular Weight Olfactory Mucosa - chemistry Phosphorus Radioisotopes Rabbits Rats Rats, Wistar Turbinates
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Zusammenfassung:	The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with α-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45-kDa protein is about 2% of the total soluble proteins of the olfactory mucosa and it is located at the mucociliary surface. According to photo-affinity labeling, the 45-kDa protein possesses a high affinity to GTP and exhibits low GTP hydrolytic activity. The functions of the 45-kDa protein are discussed. Chem, Senses 21: 181–188, 1996.
ISSN:	0379-864X 1464-3553
DOI:	10.1093/chemse/21.2.181