Circulating immunoreactive and bioactive follicle stimulating hormone concentrations in anovulatory infertile women and during gonadotrophin induction of ovulation using a decremental dose regimen

Our purpose was to determine whether decreased follicle stimulating hormone (FSH) activity, either systemic or at the follicular level, is involved in impaired follicle growth associated with normogonadotrophic anovulation. To differentiate between the possible levels of disturbance, bioactive (BIO-FSH; using the in-vitro rat granulosa cell aromatase bioassay) and immunoreactive (IRMA-FSH) FSH serum concentrations of three groups of subjects were compared: (i) 172 normogonadotrophic anovulatory infertile women during baseline conditions, (ii) 22 clomi-phene-resistant polycystic ovary syndrome patients undergoing ovulation induction by exogenous gonadotrophins using a decremental dose regimen, and (iii) nine regularly cycling controls. BIO-FSH [13.2 (range 0.8–29.5) IU/1] and IRMA-FSH [4.4 (range 1.2–9.3) IU/1] concentrations in anovulatory women during baseline conditions were significantly lower than maximum concentrations reached during the follicular phase in controls [18.7 (13.2–23.4) and 6.4 (5.7–10.0) IU/1 respectively], but were not significantly different from initial concentrations in controls [10.4 (7.2–19.6) and 4.8 (2.8–8.2) IU/1 respectively]. Moreover, concentrations of IRMA-FSH and BIO-FSH were negatively correlated (r = −0.25, P = 0.01, and r = −0.24, P = 0.02 respectively) with the interval between last vaginal bleeding and blood sampling. Maximum concentrations of IRMA-FSH [7.6 (3.9–10.9) IU/1] during ovulation induction by gonadotrophins were not significantly different from [6.4 (5.7–10.0) IU/1] concentrations in controls, whereas maximum BIO-FSH concentrations [13.5 (8.7–17.4) versus 18.7 (13.2–23.4) IU/1] were significantly lower. Our findings suggest that (i) circulating FSH does not reach concentrations that are sufficient to induce normal follicle development in anovulatory women during base-line conditions, and (ii) the FSH threshold for ovarian stimulation of this patient group is not different from normal