Establishment and characterization of a human urinary bladder carcinoma cell line (TSGH-8301)

A cell line derived from a well‐differentiated human transitional cell carcinoma of the urinary bladder, designated TSGH‐8301, was established in vitro. The cultured epithelioid cells exhibited monolayer growth and loss of contact inhibition. The tumorigenicity of TSGH‐8301 had been shown by growth in soft agar and tumor induction in athymic nude mice. A reverse ratio of lactate dehydrogenase (LHD) isoenzyme in the cell line and nude mouse‐grown tumors was seen predominantly with LDH‐V. Chromosomal analysis revealed a heterodiploid stem line with a modal number of 50. Sera of urinary bladder cancer patients reacted with membrane antigens of the TSGH‐8301 cells, suggesting the existence of tumor‐associated antigens in the cells. In vitro chemosensitivity tests of these cells may provide data valuable in the selection of proper anticancer drugs for the TSGH‐8301 donor patient.