A galactose specific agglutinin from the hemolymph of the snail Achatina fulica: purification and characterization
A galactose specific agglutinin has been detected in the hemolymph of the snail Achatina fulica fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purifie...
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| Veröffentlicht in: | Developmental and comparative immunology 1988, Vol.12 (1), p.33-42 |
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| container_title | Developmental and comparative immunology |
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| creator | Mitra, D Sarkar, M |
| description | A galactose specific agglutinin has been detected in the hemolymph of the snail Achatina fulica fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purified agglutinin has a native molecular weight of 210,000 and comprises of non covalently linked identical subunits of molecular weight 15,000. The pI of the agglutinin was found to be 8.1. The activity of the agglutinin is independent of divalent cations. The inhibition of hemagglutination data reveals that 2-deoxy-D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose. |
| doi_str_mv | 10.1016/0145-305X(88)90022-5 |
| format | Article |
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The inhibition of hemagglutination data reveals that 2-deoxy-D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/0145-305X(88)90022-5</identifier><identifier>PMID: 3350191</identifier><identifier>CODEN: DCIMDQ</identifier><language>eng</language><publisher>Oxford: Elsevier Science</publisher><subject>Achatina fulica ; Animals ; Biological and medical sciences ; Chromatography, Affinity ; Chromatography, Gel ; Fundamental and applied biological sciences. 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The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purified agglutinin has a native molecular weight of 210,000 and comprises of non covalently linked identical subunits of molecular weight 15,000. The pI of the agglutinin was found to be 8.1. The activity of the agglutinin is independent of divalent cations. The inhibition of hemagglutination data reveals that 2-deoxy-D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose.</description><subject>Achatina fulica</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Affinity</subject><subject>Chromatography, Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galectins</subject><subject>Hemagglutination Inhibition Tests</subject><subject>Hemagglutinins - isolation & purification</subject><subject>Hemolymph - immunology</subject><subject>Invertebrates</subject><subject>Isoelectric Point</subject><subject>Molecular Weight</subject><subject>Mollusca</subject><subject>Protein Conformation</subject><subject>Snails - immunology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkclOBCEYhInR6Li8gSYcjNFDK0tDg7eJcUtMvGjijdAMzGDoReg-6NNLj5O5yoWk_o8CqgA4xegaI8xvEC5ZQRH7uBTiSiJESMF2wAyLShYICbkLZlvkABym9InyEhjtg31KGcISz0Ccw6UO2gxdsjD11njnDdTLZRgH3_oWutg1cFhZuLJNF76bfgU7txZSq32Ac7PSmdTQjcEbfQv7MU4eWexaqNsFzEDMF9jof9biMdhzOiR7stmPwPvD_dvdU_Hy-vh8N38pDOVkKEpOqLNYMGakrDhhVnAmCEEcy7piREiMjNQ1K3ltqWP5_5oRzlC9WDCLGT0CF3--fey-RpsG1fhkbAi6td2YVCVyOpJX_4K4FFSUmGSw_ANN7FKK1qk--kbHb4WRmjpRU-BqClwJodadqOkhZxv_sW7sYntoU0Ken2_mOhkdXNSt8WmLVZyWlAj6C-I4k_M</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Mitra, D</creator><creator>Sarkar, M</creator><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1988</creationdate><title>A galactose specific agglutinin from the hemolymph of the snail Achatina fulica: purification and characterization</title><author>Mitra, D ; Sarkar, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-4623fe1855c997625e8658220619b7528910c9ab546be3f5022a52650bdd5e153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Achatina fulica</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Affinity</topic><topic>Chromatography, Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galectins</topic><topic>Hemagglutination Inhibition Tests</topic><topic>Hemagglutinins - isolation & purification</topic><topic>Hemolymph - immunology</topic><topic>Invertebrates</topic><topic>Isoelectric Point</topic><topic>Molecular Weight</topic><topic>Mollusca</topic><topic>Protein Conformation</topic><topic>Snails - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitra, D</creatorcontrib><creatorcontrib>Sarkar, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitra, D</au><au>Sarkar, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A galactose specific agglutinin from the hemolymph of the snail Achatina fulica: purification and characterization</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>1988</date><risdate>1988</risdate><volume>12</volume><issue>1</issue><spage>33</spage><epage>42</epage><pages>33-42</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><coden>DCIMDQ</coden><abstract>A galactose specific agglutinin has been detected in the hemolymph of the snail Achatina fulica fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purified agglutinin has a native molecular weight of 210,000 and comprises of non covalently linked identical subunits of molecular weight 15,000. The pI of the agglutinin was found to be 8.1. The activity of the agglutinin is independent of divalent cations. The inhibition of hemagglutination data reveals that 2-deoxy-D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose.</abstract><cop>Oxford</cop><pub>Elsevier Science</pub><pmid>3350191</pmid><doi>10.1016/0145-305X(88)90022-5</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0145-305X |
| ispartof | Developmental and comparative immunology, 1988, Vol.12 (1), p.33-42 |
| issn | 0145-305X 1879-0089 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Achatina fulica Animals Biological and medical sciences Chromatography, Affinity Chromatography, Gel Fundamental and applied biological sciences. Psychology Galectins Hemagglutination Inhibition Tests Hemagglutinins - isolation & purification Hemolymph - immunology Invertebrates Isoelectric Point Molecular Weight Mollusca Protein Conformation Snails - immunology |
| title | A galactose specific agglutinin from the hemolymph of the snail Achatina fulica: purification and characterization |
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