A galactose specific agglutinin from the hemolymph of the snail Achatina fulica: purification and characterization

A galactose specific agglutinin has been detected in the hemolymph of the snail Achatina fulica fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purifie...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Developmental and comparative immunology 1988, Vol.12 (1), p.33-42
Hauptverfasser:	Mitra, D, Sarkar, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Achatina fulica Animals Biological and medical sciences Chromatography, Affinity Chromatography, Gel Fundamental and applied biological sciences. Psychology Galectins Hemagglutination Inhibition Tests Hemagglutinins - isolation & purification Hemolymph - immunology Invertebrates Isoelectric Point Molecular Weight Mollusca Protein Conformation Snails - immunology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A galactose specific agglutinin has been detected in the hemolymph of the snail Achatina fulica fulica Bowdich. The agglutinin, which agglutinates rabbit erythrocytes, has been purified by affinity chromatography on crosslinked guargum followed by gel filtration on a Biogel P-200 column. The purified agglutinin has a native molecular weight of 210,000 and comprises of non covalently linked identical subunits of molecular weight 15,000. The pI of the agglutinin was found to be 8.1. The activity of the agglutinin is independent of divalent cations. The inhibition of hemagglutination data reveals that 2-deoxy-D-galactose is the most potent inhibitor of the agglutinin followed by galactose and lactose.
ISSN:	0145-305X 1879-0089
DOI:	10.1016/0145-305X(88)90022-5