Crystallization and preliminary crystallographic data for oncomodulin

Crystallization and preliminary crystallographic data for oncomodulin

Oncomodulin, a parvalbumin-like calcium-binding protein isolated from rat tumours, has been crystallized from 33% polyethylene glycol 6000 at pH 5·2 in the presence of CaCl 2 and dithiothreitol. The crystals belong to the space group P2 12 12 1, with unit cell dimensions a = 39·59(1) A ̊ , b = 64·28...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of molecular biology 1988-01, Vol.199 (2), p.393-394
Hauptverfasser: Przybylska, Maria, Ahmed, Farid R., Birnbaum, George I., Rose, David R.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Calcium-Binding Proteins
Crystalline structure
Crystallization
Crystallography
Fundamental and applied biological sciences. Psychology
Molecular biophysics
Rats
Structure in molecular biology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Oncomodulin, a parvalbumin-like calcium-binding protein isolated from rat tumours, has been crystallized from 33% polyethylene glycol 6000 at pH 5·2 in the presence of CaCl 2 and dithiothreitol. The crystals belong to the space group P2 12 12 1, with unit cell dimensions a = 39·59(1) A ̊ , b = 64·28(2) A ̊ and c = 33·07(1) A ̊ , and have one molecule of oncomodulin per asymmetric unit. Their solvent content is only 31% ( v v ) and they are remarkably stable. Three complete sets of data, one to 1·85 Å resolution for the native protein and one to 2·0 Å for each of two heavy-atom derivatives, have been collected. A three-dimensional structure analysis is in progress.
ISSN:0022-2836
1089-8638
DOI:10.1016/0022-2836(88)90324-5