Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation
The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [ 3H]arachidonic ac...
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| Veröffentlicht in: | FEBS letters 1996-06, Vol.388 (2), p.238-241 |
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| creator | Korystov, Yuri N. Dobrovinskaya, Oksana R. Shaposhnikova, Vera V. Eidus, Lazar Kh |
| description | The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [
3H]arachidonic acid-labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis. |
| doi_str_mv | 10.1016/0014-5793(96)00538-8 |
| format | Article |
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3H]arachidonic acid-labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(96)00538-8</identifier><identifier>PMID: 8690095</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid ; 4-bromophenacylbromide ; A23187 ; Animals ; Apoptosis ; arachidonic acid ; Arachidonic Acid - metabolism ; BPB ; calcium ionophore ; Cyclooxygenase Inhibitors - pharmacology ; dimethyl sulfoxide ; DMSO ; DNA Damage ; EDTA ; ethylene diamine tetraacetic acid ; Gamma Rays ; HEPES ; Irradiation ; Lipoxygenase - metabolism ; Lipoxygenase Inhibitors - pharmacology ; Male ; Masoprocol - pharmacology ; NDGA ; nordihydroguaiaretic acid ; phospholipase A2 ; PLA2 ; Prostaglandin-Endoperoxide Synthases - metabolism ; Rats ; Rats, Wistar ; Thymocyte apoptosis ; Thymus Gland - cytology ; Thymus Gland - metabolism ; Thymus Gland - radiation effects</subject><ispartof>FEBS letters, 1996-06, Vol.388 (2), p.238-241</ispartof><rights>1996</rights><rights>FEBS Letters 388 (1996) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4518-806131e9c0cfea1561181a1139b4b0b745eee11e136eae3e65e5fe281e17ef2a3</citedby><cites>FETCH-LOGICAL-c4518-806131e9c0cfea1561181a1139b4b0b745eee11e136eae3e65e5fe281e17ef2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2F0014-5793%2896%2900538-8$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(96)00538-8$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,3550,27924,27925,45574,45575,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8690095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Korystov, Yuri N.</creatorcontrib><creatorcontrib>Dobrovinskaya, Oksana R.</creatorcontrib><creatorcontrib>Shaposhnikova, Vera V.</creatorcontrib><creatorcontrib>Eidus, Lazar Kh</creatorcontrib><title>Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [
3H]arachidonic acid-labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis.</description><subject>4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid</subject><subject>4-bromophenacylbromide</subject><subject>A23187</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>arachidonic acid</subject><subject>Arachidonic Acid - metabolism</subject><subject>BPB</subject><subject>calcium ionophore</subject><subject>Cyclooxygenase Inhibitors - pharmacology</subject><subject>dimethyl sulfoxide</subject><subject>DMSO</subject><subject>DNA Damage</subject><subject>EDTA</subject><subject>ethylene diamine tetraacetic acid</subject><subject>Gamma Rays</subject><subject>HEPES</subject><subject>Irradiation</subject><subject>Lipoxygenase - metabolism</subject><subject>Lipoxygenase Inhibitors - pharmacology</subject><subject>Male</subject><subject>Masoprocol - pharmacology</subject><subject>NDGA</subject><subject>nordihydroguaiaretic acid</subject><subject>phospholipase A2</subject><subject>PLA2</subject><subject>Prostaglandin-Endoperoxide Synthases - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Thymocyte apoptosis</subject><subject>Thymus Gland - cytology</subject><subject>Thymus Gland - metabolism</subject><subject>Thymus Gland - radiation effects</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF9L7DAQxYNc8a5_voEX8iT6UM1s2jR5EXRxVVgQRJ9Dmk4x0jZr0lX225u6i496n4aZc-bM8CPkGNg5MBAXjEGeFaXip0qcMVZwmckdMgFZ8oznQv4hk2_LX7If4ytLvQS1R_akUIypYkIWj75F6htqgrEvrva9s9RYV9MOB1P51sWOup4OL-vO2_WA1Cz9cvDRRWqaAQN1IZjamcH5_pDsNqaNeLStB-R5fvM0u8sWD7f3s6tFZvMC0pdMAAdUltkGDRQC0lcGgKsqr1hV5gUiAiBwgQY5igKLBqcyTUpspoYfkJNN7jL4txXGQXcuWmxb06NfRV1KyJlg-a_GdFsllCoZ843RBh9jwEYvg-tMWGtgeqStR5R6RKnV2CTaWqa1f9v8VdVh_b20xZv0-Ub_cC2u_ytTz2-up6MwzpX4mo6HLjdBmLC-Oww6Woe9xdoFtIOuvfv50093EqGp</recordid><startdate>19960617</startdate><enddate>19960617</enddate><creator>Korystov, Yuri N.</creator><creator>Dobrovinskaya, Oksana R.</creator><creator>Shaposhnikova, Vera V.</creator><creator>Eidus, Lazar Kh</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960617</creationdate><title>Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation</title><author>Korystov, Yuri N. ; Dobrovinskaya, Oksana R. ; Shaposhnikova, Vera V. ; Eidus, Lazar Kh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4518-806131e9c0cfea1561181a1139b4b0b745eee11e136eae3e65e5fe281e17ef2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid</topic><topic>4-bromophenacylbromide</topic><topic>A23187</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>arachidonic acid</topic><topic>Arachidonic Acid - metabolism</topic><topic>BPB</topic><topic>calcium ionophore</topic><topic>Cyclooxygenase Inhibitors - pharmacology</topic><topic>dimethyl sulfoxide</topic><topic>DMSO</topic><topic>DNA Damage</topic><topic>EDTA</topic><topic>ethylene diamine tetraacetic acid</topic><topic>Gamma Rays</topic><topic>HEPES</topic><topic>Irradiation</topic><topic>Lipoxygenase - metabolism</topic><topic>Lipoxygenase Inhibitors - pharmacology</topic><topic>Male</topic><topic>Masoprocol - pharmacology</topic><topic>NDGA</topic><topic>nordihydroguaiaretic acid</topic><topic>phospholipase A2</topic><topic>PLA2</topic><topic>Prostaglandin-Endoperoxide Synthases - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Thymocyte apoptosis</topic><topic>Thymus Gland - cytology</topic><topic>Thymus Gland - metabolism</topic><topic>Thymus Gland - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Korystov, Yuri N.</creatorcontrib><creatorcontrib>Dobrovinskaya, Oksana R.</creatorcontrib><creatorcontrib>Shaposhnikova, Vera V.</creatorcontrib><creatorcontrib>Eidus, Lazar Kh</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Korystov, Yuri N.</au><au>Dobrovinskaya, Oksana R.</au><au>Shaposhnikova, Vera V.</au><au>Eidus, Lazar Kh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-06-17</date><risdate>1996</risdate><volume>388</volume><issue>2</issue><spage>238</spage><epage>241</epage><pages>238-241</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [
3H]arachidonic acid-labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8690095</pmid><doi>10.1016/0014-5793(96)00538-8</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1996-06, Vol.388 (2), p.238-241 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | 4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid 4-bromophenacylbromide A23187 Animals Apoptosis arachidonic acid Arachidonic Acid - metabolism BPB calcium ionophore Cyclooxygenase Inhibitors - pharmacology dimethyl sulfoxide DMSO DNA Damage EDTA ethylene diamine tetraacetic acid Gamma Rays HEPES Irradiation Lipoxygenase - metabolism Lipoxygenase Inhibitors - pharmacology Male Masoprocol - pharmacology NDGA nordihydroguaiaretic acid phospholipase A2 PLA2 Prostaglandin-Endoperoxide Synthases - metabolism Rats Rats, Wistar Thymocyte apoptosis Thymus Gland - cytology Thymus Gland - metabolism Thymus Gland - radiation effects |
| title | Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation |
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