Role of arachidonic acid metabolism in thymocyte apoptosis after irradiation

The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [ 3H]arachidonic ac...

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Veröffentlicht in:	FEBS letters 1996-06, Vol.388 (2), p.238-241
Hauptverfasser:	Korystov, Yuri N., Dobrovinskaya, Oksana R., Shaposhnikova, Vera V., Eidus, Lazar Kh
Format:	Artikel
Sprache:	eng
Schlagworte:	4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid 4-bromophenacylbromide A23187 Animals Apoptosis arachidonic acid Arachidonic Acid - metabolism BPB calcium ionophore Cyclooxygenase Inhibitors - pharmacology dimethyl sulfoxide DMSO DNA Damage EDTA ethylene diamine tetraacetic acid Gamma Rays HEPES Irradiation Lipoxygenase - metabolism Lipoxygenase Inhibitors - pharmacology Male Masoprocol - pharmacology NDGA nordihydroguaiaretic acid phospholipase A2 PLA2 Prostaglandin-Endoperoxide Synthases - metabolism Rats Rats, Wistar Thymocyte apoptosis Thymus Gland - cytology Thymus Gland - metabolism Thymus Gland - radiation effects
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Zusammenfassung:	The present study demonstrates that DNA fragmentation, nuclear pycnosis and trypan blue staining of irradiated thymocytes is prevented by inhibition of the lipoxygenase pathway of arachidonic acid metabolism and is not affected by cyclooxygenase inhibition. Exposed to irradiation [ 3H]arachidonic acid-labeled thymocytes release radioactive products to the external medium. The process is blocked by the lipoxygenase inhibitor, nordihydroguaiaretic acid. Thus, it can be concluded that irradiation activates arachidonic acid metabolism and that lipoxygenase metabolites play an important role in thymocyte apoptosis.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(96)00538-8