Determination of the enantiomeric composition of mexiletine and its four hydroxylated metabolites in urine by enantioselective capillary gas chromatography

Determination of the enantiomeric composition of mexiletine and its four hydroxylated metabolites in urine by enantioselective capillary gas chromatography

The enantiomers of mexiletine and four of its hydroxylated metabolites were directly separated by capillary gas chromatography using a heptakis(6‐O‐tert‐butyl‐dimethylsilyl‐2,3‐di‐O‐methyl)‐β‐cyclodextrin column. The method was applied to the analysis of urine samples from cancer patients who were t...

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Veröffentlicht in:Chirality (New York, N.Y.) N.Y.), 1996, Vol.8 (1), p.30-34
Hauptverfasser: Knoche, Beate, Gehrcke, Bärbel, König, Wilfried A., Wainer, Irving W.
Format: Artikel
Sprache:eng
Schlagworte:
assay
chiral separation
Chromatography, Gas
conjugation/deconjugation
gas chromatography
Humans
hydroxylated metabolites
Hydroxylation
mexiletine
Mexiletine - chemistry
Mexiletine - pharmacokinetics
Mexiletine - urine
modified β-cyclodextrin column
Stereoisomerism
urine
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Zusammenfassung:The enantiomers of mexiletine and four of its hydroxylated metabolites were directly separated by capillary gas chromatography using a heptakis(6‐O‐tert‐butyl‐dimethylsilyl‐2,3‐di‐O‐methyl)‐β‐cyclodextrin column. The method was applied to the analysis of urine samples from cancer patients who were treated with racemic mexiletine as part of a study of the use of mexiletine in the relief of neuropathic pain. Samples analyzed before and after deconjugation of the urine with β‐glucuronidase/arylsulfatase showed a high stereoselectivity in the formation and conjugation of these compounds. © 1996 Wiley‐Liss, Inc.
ISSN:0899-0042
1520-636X
DOI:10.1002/(SICI)1520-636X(1996)8:1<30::AID-CHIR7>3.0.CO;2-K