Purification of the LSTRA tyrosine protein kinase (p56lck)

p56lck, a p60src related tyrosine protein kinase was recently described in a murine lymphoma cell line (LSTRA), in several human lymphomas and in normal peripheral lymphocytes. We have purifed p56lck to homogeneity by electrofocusing followed by two‐step SDS/polyacrylamide gel electrophoresis. The p...

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Veröffentlicht in:	European journal of biochemistry 1988-02, Vol.172 (1), p.67-72
Hauptverfasser:	TAN, Huynh, FISCHER, Siegmund, FAGARD, Remi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - isolation & purification Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Line Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Isoelectric Focusing Leukemia, Experimental - enzymology Lymphocytes - enzymology Lymphoma - enzymology Mice Phosphorylation Protein-Tyrosine Kinases - isolation & purification Transferases
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Beschreibung
Zusammenfassung:	p56lck, a p60src related tyrosine protein kinase was recently described in a murine lymphoma cell line (LSTRA), in several human lymphomas and in normal peripheral lymphocytes. We have purifed p56lck to homogeneity by electrofocusing followed by two‐step SDS/polyacrylamide gel electrophoresis. The pure protein was identical to the p56 characterized in LSTRA cells and used to raise antibodies in rabbits. This simple procedure is applicable for the rapid purification of minor proteins.
ISSN:	0014-2956 1432-1033
DOI:	10.1111/j.1432-1033.1988.tb13856.x