Circulating Leptin Levels Are Modulated by Fasting, Cold Exposure and Insulin Administration in Lean but Not Zucker (fa/fa) Rats as Measured by ELISA
The development of a sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats. Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p
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| Veröffentlicht in: | Biochemical and biophysical research communications 1996-06, Vol.223 (3), p.660-665 |
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| container_title | Biochemical and biophysical research communications |
| container_volume | 223 |
| creator | Hardie, Laura J. Rayner, D.Vernon Holmes, Stephen Trayhurn, Paul |
| description | The development of a sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats. Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p |
| doi_str_mv | 10.1006/bbrc.1996.0951 |
| format | Article |
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Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p<0.01) less was found in the plasma ofob/obmice. In lean Zucker rats leptin circulated at approximately 4ng/ml plasma whereas levels were elevated more than 6-fold infa/farats. Circulating leptin levels declined (p<0.05) in lean rats which were fasted or exposed to 4°C for 24h, but subsequently recovered to normal within 12h of refeeding or warming, respectively. Administration of insulin increased leptin levels in lean rats within 4h (p<0.01). However, leptin levels were unchanged infa/farats exposed to the same physiological stimuli.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1996.0951</identifier><identifier>PMID: 8687452</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acclimatization ; Animals ; Cold Temperature ; Eating ; Enzyme-Linked Immunosorbent Assay ; Fasting ; Insulin - pharmacology ; Leptin ; Male ; Mice ; Mice, Obese ; Obesity - blood ; Proteins - analysis ; Proteins - drug effects ; Proteins - metabolism ; Rats ; Rats, Zucker ; Thinness</subject><ispartof>Biochemical and biophysical research communications, 1996-06, Vol.223 (3), p.660-665</ispartof><rights>1996 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c339t-71d555a609b58ced2bd0d13405272fb1602c35a9e009afc007a7e9f4717aaa1b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1996.0951$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8687452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hardie, Laura J.</creatorcontrib><creatorcontrib>Rayner, D.Vernon</creatorcontrib><creatorcontrib>Holmes, Stephen</creatorcontrib><creatorcontrib>Trayhurn, Paul</creatorcontrib><title>Circulating Leptin Levels Are Modulated by Fasting, Cold Exposure and Insulin Administration in Lean but Not Zucker (fa/fa) Rats as Measured by ELISA</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The development of a sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats. Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p<0.01) less was found in the plasma ofob/obmice. In lean Zucker rats leptin circulated at approximately 4ng/ml plasma whereas levels were elevated more than 6-fold infa/farats. Circulating leptin levels declined (p<0.05) in lean rats which were fasted or exposed to 4°C for 24h, but subsequently recovered to normal within 12h of refeeding or warming, respectively. Administration of insulin increased leptin levels in lean rats within 4h (p<0.01). However, leptin levels were unchanged infa/farats exposed to the same physiological stimuli.</description><subject>Acclimatization</subject><subject>Animals</subject><subject>Cold Temperature</subject><subject>Eating</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fasting</subject><subject>Insulin - pharmacology</subject><subject>Leptin</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Obese</subject><subject>Obesity - blood</subject><subject>Proteins - analysis</subject><subject>Proteins - drug effects</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Zucker</subject><subject>Thinness</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi1EVZbClRuSTwikZjtO4iQ-rlZbWGkLEi0S4mJN7AkyZOPFTir6ILwvTnfFjdMc_m8-y_Mz9krAUgBUV20bzFIoVS1BSfGELQQoyHIB5VO2gERkuRJfn7HnMf4AEKKs1Dk7b6qmLmW-YH_WLpipx9EN3_mODmmmcU995KtA_MbbOSTL2wd-jXHGLvna95Zvfh98nBKDg-XbIU59Wl3ZvRtcHEMS-oE_ynDg7TTyj37k3ybzkwJ_2-FVh-_4Zxwjx8hvCGfT4yOb3fZ29YKdddhHenmaF-zL9eZu_SHbfXq_Xa92mSkKNWa1sFJKrEC1sjFk89aCFUUJMq_zrhUV5KaQqAhAYWcAaqxJdWUtakQUbXHB3hy9h-B_TRRHvXfRUN_jQH6Kum5EXiglE7g8gib4GAN1-hDcHsODFqDnHvTcg5570HMPaeH1yTy1e7L_8NPhU94c83RpuncUdDSOhvQJF8iM2nr3P_VfRVuXGA</recordid><startdate>19960625</startdate><enddate>19960625</enddate><creator>Hardie, Laura J.</creator><creator>Rayner, D.Vernon</creator><creator>Holmes, Stephen</creator><creator>Trayhurn, Paul</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960625</creationdate><title>Circulating Leptin Levels Are Modulated by Fasting, Cold Exposure and Insulin Administration in Lean but Not Zucker (fa/fa) Rats as Measured by ELISA</title><author>Hardie, Laura J. ; Rayner, D.Vernon ; Holmes, Stephen ; Trayhurn, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-71d555a609b58ced2bd0d13405272fb1602c35a9e009afc007a7e9f4717aaa1b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acclimatization</topic><topic>Animals</topic><topic>Cold Temperature</topic><topic>Eating</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fasting</topic><topic>Insulin - pharmacology</topic><topic>Leptin</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Obese</topic><topic>Obesity - blood</topic><topic>Proteins - analysis</topic><topic>Proteins - drug effects</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Zucker</topic><topic>Thinness</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hardie, Laura J.</creatorcontrib><creatorcontrib>Rayner, D.Vernon</creatorcontrib><creatorcontrib>Holmes, Stephen</creatorcontrib><creatorcontrib>Trayhurn, Paul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hardie, Laura J.</au><au>Rayner, D.Vernon</au><au>Holmes, Stephen</au><au>Trayhurn, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating Leptin Levels Are Modulated by Fasting, Cold Exposure and Insulin Administration in Lean but Not Zucker (fa/fa) Rats as Measured by ELISA</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1996-06-25</date><risdate>1996</risdate><volume>223</volume><issue>3</issue><spage>660</spage><epage>665</epage><pages>660-665</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The development of a sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats. Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p<0.01) less was found in the plasma ofob/obmice. In lean Zucker rats leptin circulated at approximately 4ng/ml plasma whereas levels were elevated more than 6-fold infa/farats. Circulating leptin levels declined (p<0.05) in lean rats which were fasted or exposed to 4°C for 24h, but subsequently recovered to normal within 12h of refeeding or warming, respectively. Administration of insulin increased leptin levels in lean rats within 4h (p<0.01). However, leptin levels were unchanged infa/farats exposed to the same physiological stimuli.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8687452</pmid><doi>10.1006/bbrc.1996.0951</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1996-06, Vol.223 (3), p.660-665 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acclimatization Animals Cold Temperature Eating Enzyme-Linked Immunosorbent Assay Fasting Insulin - pharmacology Leptin Male Mice Mice, Obese Obesity - blood Proteins - analysis Proteins - drug effects Proteins - metabolism Rats Rats, Zucker Thinness |
| title | Circulating Leptin Levels Are Modulated by Fasting, Cold Exposure and Insulin Administration in Lean but Not Zucker (fa/fa) Rats as Measured by ELISA |
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