Circulating Leptin Levels Are Modulated by Fasting, Cold Exposure and Insulin Administration in Lean but Not Zucker (fa/fa) Rats as Measured by ELISA

Circulating Leptin Levels Are Modulated by Fasting, Cold Exposure and Insulin Administration in Lean but Not Zucker (fa/fa) Rats as Measured by ELISA

The development of a sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats. Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical and biophysical research communications 1996-06, Vol.223 (3), p.660-665
Hauptverfasser: Hardie, Laura J., Rayner, D.Vernon, Holmes, Stephen, Trayhurn, Paul
Format: Artikel
Sprache:eng
Schlagworte:
Acclimatization
Animals
Cold Temperature
Eating
Enzyme-Linked Immunosorbent Assay
Fasting
Insulin - pharmacology
Leptin
Male
Mice
Mice, Obese
Obesity - blood
Proteins - analysis
Proteins - drug effects
Proteins - metabolism
Rats
Rats, Zucker
Thinness
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The development of a sensitive enzyme-linked immunosorbent assay (ELISA) is described for the quantitation of plasma leptin levels in both mice and rats. Approximately 1.5ng leptin/ml plasma was detected in lean mice but significantly (p
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0951