Expression of Shigella sonnei lipopolysaccharide in Vibrio cholerae

Making use of a newly designed mobilizable suicide vector, the genetic determinants encoding Shigella sonnei lipopolysaccharide (LPS) were stably integrated into the chromosome of the live attenuated Vibrio cholerae vaccine strain CVD103‐HgR. Expression studies showed that the production of complete...

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Veröffentlicht in:Molecular microbiology 1996-03, Vol.19 (5), p.949-963
Hauptverfasser: Viret, Jean‐François, Cryz, Jr, Stanley J., Favre, Didier
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Sprache:eng
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Zusammenfassung:Making use of a newly designed mobilizable suicide vector, the genetic determinants encoding Shigella sonnei lipopolysaccharide (LPS) were stably integrated into the chromosome of the live attenuated Vibrio cholerae vaccine strain CVD103‐HgR. Expression studies showed that the production of complete S. sonnei O‐polysaccharide (O‐PS)‐bearing LPS was limited in bivalent recombinant strains that were also proficient in the synthesis of the host‐encoded Inaba O‐PS. Conversely, high amounts of LPS carrying S. sonnei O‐PS are produced in monovalent Inaba‐deficient derivatives, even in those strains which do not co‐express the compatible R1 LPS core. Thus, the non‐enterobacterial V. cholerae LPS core efficiently acts as a receptor for covalent binding of S. sonnei O‐PS provided that competition with the host O‐PS is avoided. Expression of the R1 core interferes with cell division in recombinant V. cholerae without affecting other physiological properties of vaccine strain CVD103‐HgR. Both monovalent and bivalent strains stimulated high serum‐antibody titres specific for their respective O‐serotype(s) when administered to rabbits. The potential of V. cholerae as an expression carrier for heterologous O‐serotypes is discussed.
ISSN:0950-382X
1365-2958
DOI:10.1046/j.1365-2958.1996.435967.x