Ultrafiltration discrepancies in recovery of myoglobin from urine
We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the origina...
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| Veröffentlicht in: | Clinical chemistry (Baltimore, Md.) Md.), 1996-06, Vol.42 (6), p.965-969 |
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| container_title | Clinical chemistry (Baltimore, Md.) |
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| creator | Loun, B Copeland, KR Sedor, FA |
| description | We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filtrate were assayed for myoglobin. The amount of myoglobin recovered subsequent to filtration varied from |
| doi_str_mv | 10.1093/clinchem/42.6.965 |
| format | Article |
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Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filtrate were assayed for myoglobin. The amount of myoglobin recovered subsequent to filtration varied from <1-38%. This poor and variable recovery was independent of sample matrix or precentrifugation of the specimens. This was most critical for urine specimens with myoglobin concentrations <60 000 microg/L. Fourteen of 18 such filtrates had concentrations <350 microg/L, a concentration below which a negative result would be obtained by using conventional dipstick methods. Thus, the use of this procedure has the potential to misdiagnose patients with myoglobin concentrations associated with increased risk of subsequent renal dysfunction, in particular when urine myoglobin concentrations are <60 000 microg/L.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1093/clinchem/42.6.965</identifier><identifier>PMID: 8665690</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Biological and medical sciences ; False Negative Reactions ; Humans ; Hydrogen-Ion Concentration ; Investigative techniques, diagnostic techniques (general aspects) ; Kidney Diseases - urine ; Medical sciences ; Microchemistry ; Myoglobinuria - urine ; Osteoarticular system. Muscles ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Reagent Strips ; Reproducibility of Results ; Ultrafiltration</subject><ispartof>Clinical chemistry (Baltimore, Md.), 1996-06, Vol.42 (6), p.965-969</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-e85544fa152d9aa2ec499315ed1a0ed57b8c9565788a112ea7d7e81befb57203</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3105297$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8665690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loun, B</creatorcontrib><creatorcontrib>Copeland, KR</creatorcontrib><creatorcontrib>Sedor, FA</creatorcontrib><title>Ultrafiltration discrepancies in recovery of myoglobin from urine</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filtrate were assayed for myoglobin. The amount of myoglobin recovered subsequent to filtration varied from <1-38%. This poor and variable recovery was independent of sample matrix or precentrifugation of the specimens. This was most critical for urine specimens with myoglobin concentrations <60 000 microg/L. Fourteen of 18 such filtrates had concentrations <350 microg/L, a concentration below which a negative result would be obtained by using conventional dipstick methods. Thus, the use of this procedure has the potential to misdiagnose patients with myoglobin concentrations associated with increased risk of subsequent renal dysfunction, in particular when urine myoglobin concentrations are <60 000 microg/L.</description><subject>Biological and medical sciences</subject><subject>False Negative Reactions</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Kidney Diseases - urine</subject><subject>Medical sciences</subject><subject>Microchemistry</subject><subject>Myoglobinuria - urine</subject><subject>Osteoarticular system. Muscles</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Reagent Strips</subject><subject>Reproducibility of Results</subject><subject>Ultrafiltration</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRS0EgvL4ABZIWQC7FE8Sv5YI8ZKQ2MDacpwJNXLiYrdU_XtctXTjkT1nrkeHkEugU6CqvrPejXaGw11TTflUcXZAJsBqWkrG4ZBMKKWqVNCIE3Ka0ne-NkLyY3IsOWdc0Qm5__SLaHq3ORcujEXnko04N6N1mAo3FhFt-MW4LkJfDOvw5UObX_sYhmIZ3Yjn5Kg3PuHFrp6Rj6fHj4eX8u39-fXh_q20tZKLEiVjTdMbYFWnjKnQNkrVwLADQ7FjopVWMc6ElAagQiM6gRJa7FsmKlqfkdtt7DyGnyWmhR7ypui9GTEskxaSqgpAZhC2oI0hpYi9nkc3mLjWQPXGmv63pptKc52t5ZmrXfiyHbDbT-w05f71rm-SNb6PGz1pj9VAWaVExm622Mx9zVYuok6D8T6Hgl6tVvvv_gBTKIUG</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Loun, B</creator><creator>Copeland, KR</creator><creator>Sedor, FA</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960601</creationdate><title>Ultrafiltration discrepancies in recovery of myoglobin from urine</title><author>Loun, B ; Copeland, KR ; Sedor, FA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-e85544fa152d9aa2ec499315ed1a0ed57b8c9565788a112ea7d7e81befb57203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Biological and medical sciences</topic><topic>False Negative Reactions</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Kidney Diseases - urine</topic><topic>Medical sciences</topic><topic>Microchemistry</topic><topic>Myoglobinuria - urine</topic><topic>Osteoarticular system. Muscles</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Reagent Strips</topic><topic>Reproducibility of Results</topic><topic>Ultrafiltration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loun, B</creatorcontrib><creatorcontrib>Copeland, KR</creatorcontrib><creatorcontrib>Sedor, FA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loun, B</au><au>Copeland, KR</au><au>Sedor, FA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrafiltration discrepancies in recovery of myoglobin from urine</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>1996-06-01</date><risdate>1996</risdate><volume>42</volume><issue>6</issue><spage>965</spage><epage>969</epage><pages>965-969</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filtrate were assayed for myoglobin. The amount of myoglobin recovered subsequent to filtration varied from <1-38%. This poor and variable recovery was independent of sample matrix or precentrifugation of the specimens. This was most critical for urine specimens with myoglobin concentrations <60 000 microg/L. Fourteen of 18 such filtrates had concentrations <350 microg/L, a concentration below which a negative result would be obtained by using conventional dipstick methods. Thus, the use of this procedure has the potential to misdiagnose patients with myoglobin concentrations associated with increased risk of subsequent renal dysfunction, in particular when urine myoglobin concentrations are <60 000 microg/L.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>8665690</pmid><doi>10.1093/clinchem/42.6.965</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical chemistry (Baltimore, Md.), 1996-06, Vol.42 (6), p.965-969 |
| issn | 0009-9147 1530-8561 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Biological and medical sciences False Negative Reactions Humans Hydrogen-Ion Concentration Investigative techniques, diagnostic techniques (general aspects) Kidney Diseases - urine Medical sciences Microchemistry Myoglobinuria - urine Osteoarticular system. Muscles Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reagent Strips Reproducibility of Results Ultrafiltration |
| title | Ultrafiltration discrepancies in recovery of myoglobin from urine |
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