Ultrafiltration discrepancies in recovery of myoglobin from urine

We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the origina...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 1996-06, Vol.42 (6), p.965-969
Hauptverfasser:	Loun, B, Copeland, KR, Sedor, FA
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences False Negative Reactions Humans Hydrogen-Ion Concentration Investigative techniques, diagnostic techniques (general aspects) Kidney Diseases - urine Medical sciences Microchemistry Myoglobinuria - urine Osteoarticular system. Muscles Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reagent Strips Reproducibility of Results Ultrafiltration
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Beschreibung
Zusammenfassung:	We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filtrate were assayed for myoglobin. The amount of myoglobin recovered subsequent to filtration varied from
ISSN:	0009-9147 1530-8561
DOI:	10.1093/clinchem/42.6.965