Separation of the pore-forming and cytotoxic activities from natural killer cell cytotoxic factor

The influence of Ca 2+ on the cytotoxic activity of natural killer cell cytotoxic factor (NKCF) was analyzed. When the natural killer susceptible cell line K562 was exposed to NKCF in the presence of 5 mM Ca 2+, two peaks of cell damage were found. The first peak was observed after 30–40 min of incu...

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Veröffentlicht in:	FEBS letters 1988-01, Vol.226 (2), p.261-264
Hauptverfasser:	Sashchenko, L.P., Gnuchev, N.V., Kirillova, M.A., Lukjanova, T.I., Rebizova, T.V., Revazova, E.S., Lukanidin, E.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of the immune response. Humoral and cellular immunity Animals Biological and medical sciences Calcium - pharmacology Cell Line Cell Membrane - immunology Cytotoxicity, Immunologic - drug effects Fundamental and applied biological sciences. Psychology Fundamental immunology Granular lymphocyte Humans Immunobiology Killer Cells, Natural - immunology Killer Factors, Yeast Kinetics LGLs, large granular lymphocytes Mice Mice, Nude Natural killer cell cytotoxic factor NK cells, natural killer cells NKCF, natural killer cell cytotoxic factor Organs and cells involved in the immune response Perforin Proteins - immunology
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Zusammenfassung:	The influence of Ca 2+ on the cytotoxic activity of natural killer cell cytotoxic factor (NKCF) was analyzed. When the natural killer susceptible cell line K562 was exposed to NKCF in the presence of 5 mM Ca 2+, two peaks of cell damage were found. The first peak was observed after 30–40 min of incubation as a result of pore formation on the surface of target cells. The second was a peak of cytolytic activity which appeared after 24 h of incubation. Upon dilution of the NKCF preparation, only the first peak was observed. Therefore, NKCF produced by large granular lymphocytes in response to K562 consists of different proteins and represents pore-forming and cytolytic activities.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(88)81435-2