Effect of freezing on the coupling of VIP receptors to adenylate cyclase in rat liver membranes

In fresh rat liver plasma membranes, high affinity VIP receptors were specifically labelled with [ 125I] helodermin and were well coupled to adenylate cyclase while low affinity VIP receptors were not. After freezing and thawing low affinity VIP receptors were also coupled to adenylate cyclase. This...

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Veröffentlicht in:	Life Sci.; (United States) 1988, Vol.42 (5), p.505-510
Hauptverfasser:	Robberecht, Patrick, Waelbroeck, Magali, De Neef, Philippe, Camus, Jean-Claude, Gourlet, Philippe, Christophe, Jean
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques adenylate cyclase Adenylyl Cyclases - metabolism AFFINITY ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES Binding, Competitive Biological and medical sciences BIOLOGICAL EFFECTS BIOLOGY BODY CELL CONSTITUENTS Cell Membrane - metabolism CELL MEMBRANES CRYOBIOLOGY CYCLASES DATA DAYS LIVING RADIOISOTOPES DIGESTIVE SYSTEM ELECTRON CAPTURE RADIOISOTOPES Enzyme Activation - drug effects ENZYME ACTIVITY ENZYMES EXPERIMENTAL DATA FREEZING Fundamental and applied biological sciences. Psychology GLANDS Glucagon - pharmacology Guanylyl Imidodiphosphate - pharmacology INFORMATION INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES ISOTOPE APPLICATIONS ISOTOPES LIVER Liver - metabolism Liver. Bile. Biliary tracts LYASES MAMMALS MEMBRANE PROTEINS MEMBRANES NUCLEI NUMERICAL DATA ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANS PEPTIDES Peptides - metabolism PROTEINS RADIOISOTOPES RATS RECEPTORS Receptors, Gastrointestinal Hormone - metabolism Receptors, Vasoactive Intestinal Peptide RODENTS Sodium Fluoride - pharmacology THAWING TRACER TECHNIQUES vasoactive intestinal peptide Vasoactive Intestinal Peptide - metabolism Vasoactive Intestinal Peptide - pharmacology VERTEBRATES Vertebrates: digestive system
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Zusammenfassung:	In fresh rat liver plasma membranes, high affinity VIP receptors were specifically labelled with [ 125I] helodermin and were well coupled to adenylate cyclase while low affinity VIP receptors were not. After freezing and thawing low affinity VIP receptors were also coupled to adenylate cyclase. This modification of adenylate cyclase activation was specific for the VIP response as freezing and thawing did not modify Gpp (NH)p, NaF and glucagon stimulations.
ISSN:	0024-3205 1879-0631
DOI:	10.1016/0024-3205(88)90090-2