Determination of adriamycin and its fluorescent metabolites in human plasma by HPLC

We describe a method for measuring adriamycin and its metabolites, adriamycinol and adriamycinone in plasma, using reversed phase HPLC and fluorescence detection. The lower limit of detection is approximately 1 ng/mL for each compound. An extraction technique for serum is described which is capable...

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Veröffentlicht in:	Biomedical chromatography 1987-02, Vol.2 (1), p.38-40
Hauptverfasser:	Mahdadi, R., Lhermitte, M., Lafitte, J. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid Doxorubicin - analogs & derivatives Doxorubicin - blood Humans Naphthacenes - blood Spectrometry, Fluorescence
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Beschreibung
Zusammenfassung:	We describe a method for measuring adriamycin and its metabolites, adriamycinol and adriamycinone in plasma, using reversed phase HPLC and fluorescence detection. The lower limit of detection is approximately 1 ng/mL for each compound. An extraction technique for serum is described which is capable of an almost equal recovery (>93%) of adriamycin and metabolites without interference from endogenous components of plasma and from other common drugs. Within‐day and day to day coefficients of variation are estimated.
ISSN:	0269-3879 1099-0801
DOI:	10.1002/bmc.1130020111