Stimulation of mono (ADP-ribosyl)ation by reduced extracellular calcium levels in human fibroblasts

Lowering extracellular calcium in cultures of human diploid fibroblast‐like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca2+ and was inhibited by 3‐aminobenzamide, an inhibitor of ADP‐ribosyl transfer reactions. The concentrations of 3‐aminob...

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Veröffentlicht in:	Journal of cellular physiology 1988-01, Vol.134 (1), p.161-165
Hauptverfasser:	Duncan, Michael R., Rankin, Patrick R., King, Robert L., Jacobson, Myron K., Dell'Orco, Robert T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate Ribose - metabolism Biological and medical sciences Calcium - metabolism Cell metabolism, cell oxidation Cell physiology Cells, Cultured Extracellular Space - metabolism Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Homeostasis Humans Male Molecular and cellular biology NAD - metabolism Skin - cytology Skin - metabolism Stimulation, Chemical
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Zusammenfassung:	Lowering extracellular calcium in cultures of human diploid fibroblast‐like cells caused a rapid depletion of NAD pools. This loss of NAD was reversed by restoring extracellular Ca2+ and was inhibited by 3‐aminobenzamide, an inhibitor of ADP‐ribosyl transfer reactions. The concentrations of 3‐aminobenzamide needed to inhibit the loss of NAD were consistent with those required to inhibit cellular mono(ADP‐ribosyl) rather than poly(ADP‐ribosyl) reactions. Calcium depletion did not inhibit the biosynthesis of NAD. These results suggest that mono(ADP‐ribosyl)ation is involved in the regulation of cellular Ca2+ levels.
ISSN:	0021-9541 1097-4652
DOI:	10.1002/jcp.1041340121