Functional impairment of bronchoalveolar lavage phospholipids in early Pneumocystis carinii pneumonia in rats
Surfactant abnormalities may contribute to the impairment of gas exchange observed in Pneumocystis carinii pneumonia. Analysis of rat bronchoalveolar lavage (BAL) lipid extracts from normal controls, steroid controls, trimethaprim-sulfamethoxazole (TMPSMX) controls, TMP-SMX/ P. carinii pneumonia con...
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| Veröffentlicht in: | The Journal of laboratory and clinical medicine 1996-03, Vol.127 (3), p.263-271 |
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| container_title | The Journal of laboratory and clinical medicine |
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| creator | Su, Tony H. Natarajan, Viswanathan Kachel, Diane L. Moxley, Michael A. Longmore, William J. Martin, William J. |
| description | Surfactant abnormalities may contribute to the impairment of gas exchange observed in
Pneumocystis carinii pneumonia. Analysis of rat bronchoalveolar lavage (BAL) lipid extracts from normal controls, steroid controls, trimethaprim-sulfamethoxazole (TMPSMX) controls, TMP-SMX/
P. carinii pneumonia controls, and
P. carinii pneumonia animals reveal similar total phospholipid and total protein levels. However, there was a marked reduction in phosphatidylglycerol (PG) from the BAL of
P. carinii pneumonia rats as compared with control animals, with a decrease from 4.91 ± 1.29 nmol/mg protein to 0.46 ± 0.57 nmol/mg protein (
p < 0.05) and a decrease, as a percent of total phospholipids, from 7.7% ± 0.88% to 0.91% ± 0.59% (
p < 0.001). Furthermore, in vitro surface activities of BAL lipid extracts from control and
P. carinii pneumonia rats revealed minimum surface tension increases from 9.38 ± 1.71 mN/m in controls to 16.36 ± 0.83 mN/m in
P. carinii pneumonia rats (
p < 0.05) and likewise maximum surface tension increases from 22.14 ± 4.34 mN/m to 38.57 ± 2.07 mN/m (
p < 0.01). Of interest, the surface activity of PG-deficient
P. carinii pneumonia BAL lipid extracts is completely restored to that of normal controls by the addition of exogenous PG. These findings suggest that a functionally abnormal surfactant occurs in
P. carinii pneumonia and that this may account, in part, for the impairment of gas exchange observed in this disorder. |
| doi_str_mv | 10.1016/S0022-2143(96)90094-3 |
| format | Article |
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Pneumocystis carinii pneumonia. Analysis of rat bronchoalveolar lavage (BAL) lipid extracts from normal controls, steroid controls, trimethaprim-sulfamethoxazole (TMPSMX) controls, TMP-SMX/
P. carinii pneumonia controls, and
P. carinii pneumonia animals reveal similar total phospholipid and total protein levels. However, there was a marked reduction in phosphatidylglycerol (PG) from the BAL of
P. carinii pneumonia rats as compared with control animals, with a decrease from 4.91 ± 1.29 nmol/mg protein to 0.46 ± 0.57 nmol/mg protein (
p < 0.05) and a decrease, as a percent of total phospholipids, from 7.7% ± 0.88% to 0.91% ± 0.59% (
p < 0.001). Furthermore, in vitro surface activities of BAL lipid extracts from control and
P. carinii pneumonia rats revealed minimum surface tension increases from 9.38 ± 1.71 mN/m in controls to 16.36 ± 0.83 mN/m in
P. carinii pneumonia rats (
p < 0.05) and likewise maximum surface tension increases from 22.14 ± 4.34 mN/m to 38.57 ± 2.07 mN/m (
p < 0.01). Of interest, the surface activity of PG-deficient
P. carinii pneumonia BAL lipid extracts is completely restored to that of normal controls by the addition of exogenous PG. These findings suggest that a functionally abnormal surfactant occurs in
P. carinii pneumonia and that this may account, in part, for the impairment of gas exchange observed in this disorder.</description><identifier>ISSN: 0022-2143</identifier><identifier>EISSN: 1532-6543</identifier><identifier>DOI: 10.1016/S0022-2143(96)90094-3</identifier><identifier>PMID: 9273359</identifier><identifier>CODEN: JLCMAK</identifier><language>eng</language><publisher>Saint Louis, MO: Mosby, Inc</publisher><subject>Animals ; Biological and medical sciences ; Bronchoalveolar Lavage Fluid - chemistry ; Experimental protozoal diseases and models ; Female ; Immunosuppression ; Infectious diseases ; Lipids - analysis ; Medical sciences ; Parasitic diseases ; Phosphatidylglycerols - deficiency ; Phosphatidylglycerols - pharmacology ; Phospholipids - analysis ; Phospholipids - physiology ; Pneumonia, Pneumocystis - physiopathology ; Protozoal diseases ; Rats ; Rats, Inbred Lew ; Time Factors</subject><ispartof>The Journal of laboratory and clinical medicine, 1996-03, Vol.127 (3), p.263-271</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-33bf52f3853ebb38af6708cce61dd4f19a9929c27287cedb445c80f29174cae53</citedby><cites>FETCH-LOGICAL-c389t-33bf52f3853ebb38af6708cce61dd4f19a9929c27287cedb445c80f29174cae53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3017489$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9273359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Tony H.</creatorcontrib><creatorcontrib>Natarajan, Viswanathan</creatorcontrib><creatorcontrib>Kachel, Diane L.</creatorcontrib><creatorcontrib>Moxley, Michael A.</creatorcontrib><creatorcontrib>Longmore, William J.</creatorcontrib><creatorcontrib>Martin, William J.</creatorcontrib><title>Functional impairment of bronchoalveolar lavage phospholipids in early Pneumocystis carinii pneumonia in rats</title><title>The Journal of laboratory and clinical medicine</title><addtitle>J Lab Clin Med</addtitle><description>Surfactant abnormalities may contribute to the impairment of gas exchange observed in
Pneumocystis carinii pneumonia. Analysis of rat bronchoalveolar lavage (BAL) lipid extracts from normal controls, steroid controls, trimethaprim-sulfamethoxazole (TMPSMX) controls, TMP-SMX/
P. carinii pneumonia controls, and
P. carinii pneumonia animals reveal similar total phospholipid and total protein levels. However, there was a marked reduction in phosphatidylglycerol (PG) from the BAL of
P. carinii pneumonia rats as compared with control animals, with a decrease from 4.91 ± 1.29 nmol/mg protein to 0.46 ± 0.57 nmol/mg protein (
p < 0.05) and a decrease, as a percent of total phospholipids, from 7.7% ± 0.88% to 0.91% ± 0.59% (
p < 0.001). Furthermore, in vitro surface activities of BAL lipid extracts from control and
P. carinii pneumonia rats revealed minimum surface tension increases from 9.38 ± 1.71 mN/m in controls to 16.36 ± 0.83 mN/m in
P. carinii pneumonia rats (
p < 0.05) and likewise maximum surface tension increases from 22.14 ± 4.34 mN/m to 38.57 ± 2.07 mN/m (
p < 0.01). Of interest, the surface activity of PG-deficient
P. carinii pneumonia BAL lipid extracts is completely restored to that of normal controls by the addition of exogenous PG. These findings suggest that a functionally abnormal surfactant occurs in
P. carinii pneumonia and that this may account, in part, for the impairment of gas exchange observed in this disorder.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Experimental protozoal diseases and models</subject><subject>Female</subject><subject>Immunosuppression</subject><subject>Infectious diseases</subject><subject>Lipids - analysis</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Phosphatidylglycerols - deficiency</subject><subject>Phosphatidylglycerols - pharmacology</subject><subject>Phospholipids - analysis</subject><subject>Phospholipids - physiology</subject><subject>Pneumonia, Pneumocystis - physiopathology</subject><subject>Protozoal diseases</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Time Factors</subject><issn>0022-2143</issn><issn>1532-6543</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAQgEVoSbeb_ISADqW0B7d6-KVTCaFJC4EW2pzFWB41KrLkSvbC_vvau8tecxgGZr4ZjT5Cbjj7xBmvP_9iTIhC8FJ-UPVHxZgqC3lBNrySoqirUr4imzPyhrzN-S9bIKmaS3KpRCNlpTZkuJ-DmVwM4KkbRnBpwDDRaGmXYjDPEfwOo4dEPezgD9LxOeYlvBtdn6kLFCH5Pf0ZcB6i2efJZWogueAcHQ_F4GDlEkz5iry24DNen_KWPN1__X33rXj88fD97vaxMLJVUyFlZythZVtJ7DrZgq0b1hqDNe_70nIFSgllRCPaxmDflWVlWmaF4k1pACu5Je-Pe8cU_82YJz24bNB7CBjnrJuWsZItCrakOoImxZwTWj0mN0Daa870qlkfNOvVoVa1PmjW69zN6YG5G7A_T528Lv13pz5kA94mCMblMybZcmm7Yl-OGC4ydg6TzsZhWP7kEppJ99G9cMh_MG2b9Q</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Su, Tony H.</creator><creator>Natarajan, Viswanathan</creator><creator>Kachel, Diane L.</creator><creator>Moxley, Michael A.</creator><creator>Longmore, William J.</creator><creator>Martin, William J.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>Functional impairment of bronchoalveolar lavage phospholipids in early Pneumocystis carinii pneumonia in rats</title><author>Su, Tony H. ; Natarajan, Viswanathan ; Kachel, Diane L. ; Moxley, Michael A. ; Longmore, William J. ; Martin, William J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-33bf52f3853ebb38af6708cce61dd4f19a9929c27287cedb445c80f29174cae53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Experimental protozoal diseases and models</topic><topic>Female</topic><topic>Immunosuppression</topic><topic>Infectious diseases</topic><topic>Lipids - analysis</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Phosphatidylglycerols - deficiency</topic><topic>Phosphatidylglycerols - pharmacology</topic><topic>Phospholipids - analysis</topic><topic>Phospholipids - physiology</topic><topic>Pneumonia, Pneumocystis - physiopathology</topic><topic>Protozoal diseases</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Time Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Su, Tony H.</creatorcontrib><creatorcontrib>Natarajan, Viswanathan</creatorcontrib><creatorcontrib>Kachel, Diane L.</creatorcontrib><creatorcontrib>Moxley, Michael A.</creatorcontrib><creatorcontrib>Longmore, William J.</creatorcontrib><creatorcontrib>Martin, William J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of laboratory and clinical medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Tony H.</au><au>Natarajan, Viswanathan</au><au>Kachel, Diane L.</au><au>Moxley, Michael A.</au><au>Longmore, William J.</au><au>Martin, William J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional impairment of bronchoalveolar lavage phospholipids in early Pneumocystis carinii pneumonia in rats</atitle><jtitle>The Journal of laboratory and clinical medicine</jtitle><addtitle>J Lab Clin Med</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>127</volume><issue>3</issue><spage>263</spage><epage>271</epage><pages>263-271</pages><issn>0022-2143</issn><eissn>1532-6543</eissn><coden>JLCMAK</coden><abstract>Surfactant abnormalities may contribute to the impairment of gas exchange observed in
Pneumocystis carinii pneumonia. Analysis of rat bronchoalveolar lavage (BAL) lipid extracts from normal controls, steroid controls, trimethaprim-sulfamethoxazole (TMPSMX) controls, TMP-SMX/
P. carinii pneumonia controls, and
P. carinii pneumonia animals reveal similar total phospholipid and total protein levels. However, there was a marked reduction in phosphatidylglycerol (PG) from the BAL of
P. carinii pneumonia rats as compared with control animals, with a decrease from 4.91 ± 1.29 nmol/mg protein to 0.46 ± 0.57 nmol/mg protein (
p < 0.05) and a decrease, as a percent of total phospholipids, from 7.7% ± 0.88% to 0.91% ± 0.59% (
p < 0.001). Furthermore, in vitro surface activities of BAL lipid extracts from control and
P. carinii pneumonia rats revealed minimum surface tension increases from 9.38 ± 1.71 mN/m in controls to 16.36 ± 0.83 mN/m in
P. carinii pneumonia rats (
p < 0.05) and likewise maximum surface tension increases from 22.14 ± 4.34 mN/m to 38.57 ± 2.07 mN/m (
p < 0.01). Of interest, the surface activity of PG-deficient
P. carinii pneumonia BAL lipid extracts is completely restored to that of normal controls by the addition of exogenous PG. These findings suggest that a functionally abnormal surfactant occurs in
P. carinii pneumonia and that this may account, in part, for the impairment of gas exchange observed in this disorder.</abstract><cop>Saint Louis, MO</cop><pub>Mosby, Inc</pub><pmid>9273359</pmid><doi>10.1016/S0022-2143(96)90094-3</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Bronchoalveolar Lavage Fluid - chemistry Experimental protozoal diseases and models Female Immunosuppression Infectious diseases Lipids - analysis Medical sciences Parasitic diseases Phosphatidylglycerols - deficiency Phosphatidylglycerols - pharmacology Phospholipids - analysis Phospholipids - physiology Pneumonia, Pneumocystis - physiopathology Protozoal diseases Rats Rats, Inbred Lew Time Factors |
| title | Functional impairment of bronchoalveolar lavage phospholipids in early Pneumocystis carinii pneumonia in rats |
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