Functional impairment of bronchoalveolar lavage phospholipids in early Pneumocystis carinii pneumonia in rats
Surfactant abnormalities may contribute to the impairment of gas exchange observed in Pneumocystis carinii pneumonia. Analysis of rat bronchoalveolar lavage (BAL) lipid extracts from normal controls, steroid controls, trimethaprim-sulfamethoxazole (TMPSMX) controls, TMP-SMX/ P. carinii pneumonia con...
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Veröffentlicht in: | The Journal of laboratory and clinical medicine 1996-03, Vol.127 (3), p.263-271 |
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Sprache: | eng |
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Zusammenfassung: | Surfactant abnormalities may contribute to the impairment of gas exchange observed in
Pneumocystis carinii pneumonia. Analysis of rat bronchoalveolar lavage (BAL) lipid extracts from normal controls, steroid controls, trimethaprim-sulfamethoxazole (TMPSMX) controls, TMP-SMX/
P. carinii pneumonia controls, and
P. carinii pneumonia animals reveal similar total phospholipid and total protein levels. However, there was a marked reduction in phosphatidylglycerol (PG) from the BAL of
P. carinii pneumonia rats as compared with control animals, with a decrease from 4.91 ± 1.29 nmol/mg protein to 0.46 ± 0.57 nmol/mg protein (
p < 0.05) and a decrease, as a percent of total phospholipids, from 7.7% ± 0.88% to 0.91% ± 0.59% (
p < 0.001). Furthermore, in vitro surface activities of BAL lipid extracts from control and
P. carinii pneumonia rats revealed minimum surface tension increases from 9.38 ± 1.71 mN/m in controls to 16.36 ± 0.83 mN/m in
P. carinii pneumonia rats (
p < 0.05) and likewise maximum surface tension increases from 22.14 ± 4.34 mN/m to 38.57 ± 2.07 mN/m (
p < 0.01). Of interest, the surface activity of PG-deficient
P. carinii pneumonia BAL lipid extracts is completely restored to that of normal controls by the addition of exogenous PG. These findings suggest that a functionally abnormal surfactant occurs in
P. carinii pneumonia and that this may account, in part, for the impairment of gas exchange observed in this disorder. |
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ISSN: | 0022-2143 1532-6543 |
DOI: | 10.1016/S0022-2143(96)90094-3 |