Localization of apoptotic cells by direct immunogold detection of digoxigenin-labeled genomic DNA in semithin sections

Localization of apoptotic cells by direct immunogold detection of digoxigenin-labeled genomic DNA in semithin sections

In this study, which correlates apoptosis with avian ovarian physiology, we modified an in situ DNA nick end-labeling method using immunogold reagents to detect apoptotic cells in semithin sections of quail ovaries embedded in glycol methacrylate resin. Special attention was paid to the prevention o...

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Veröffentlicht in:The journal of histochemistry and cytochemistry 1996-02, Vol.44 (2), p.183-185
Hauptverfasser: Van Nassauw, L, Schrevens, A, Harrisson, F, Callebaut, M
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Apoptosis
Coturnix
Digoxigenin - chemistry
DNA - analysis
Female
Immunohistochemistry - methods
Ovary - cytology
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Zusammenfassung:In this study, which correlates apoptosis with avian ovarian physiology, we modified an in situ DNA nick end-labeling method using immunogold reagents to detect apoptotic cells in semithin sections of quail ovaries embedded in glycol methacrylate resin. Special attention was paid to the prevention of background staining. The results are comparable with those of the ApopTag peroxidase kit in paraffin-embedded ovaries.
ISSN:0022-1554
1551-5044
DOI:10.1177/44.2.8609375