The sperm stress test: a novel test that predicts pregnancy in assisted reproductive technologies

To develop a reliable sperm test that would predict pregnancy rate in assisted reproductive technologies. Blind prospective cohort study. Tertiary-care, university hospital-affiliated IVF program. One hundred nineteen sperm samples were obtained from 110 males from couples undergoing IVF or GIFT (ART). Sperm samples were washed by Percoll, incubated at 24°C for 4hours, and an aliquot of the same sperm suspension was used for ART incubated at 40°C for 4 hours (stress test). Stress test scores are expressed as the ratio of final to initial motility. Of 119 ART cycles, 24 resulted in pregnancy. Of 24 pregnancies, 23 occurred in cycles that used sperm samples with stress test scores≥0.75 and only one with a stress test score