Characterization of the haemorrhagic enteritis virus genome and the sequence of the putative penton base and core protein genes

Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, VPI and SU, Blacksburg, VA 24061-0442, USA Haemorrhagic enteritis virus (HEV) is a member of a genetically ill-defined group within the genus Aviadenovirus which causes significant clinical disease in gallinaceous fowl. Using DNA obtained from a low virulence isolate of HEV passed in turkeys, we developed a genomic restriction map and estimated an apparent genomic length of 25·5 kb. No evidence for extensive DNA hybridization was found between the HEV genome and either the hexon or penton base genes of human adenovirus 2 (HAdV-2) and fowl adenovirus 10 (FAdV-10). The HEV penton base gene was identified by PCR using primers based on conserved adenoviral DNA sequences. The penton base gene was expressed in Escherichia coli as a fusion protein and detected by anti-HEV serum in both colony and denaturing gel immunoblots. DNA sequencing revealed a putative penton base ORF with a predicted amino acid sequence showing approximately 39·0%, 53·0% and 44·2% similarity with the penton base of HAdV-2, human adenovirus 40 (HAdV-40) and FAdV-10, respectively. The penton base gene was located at 43·3–48·6 m.u. on the HEV genome and had a remarkably low G+C content (33·8%). DNA sequencing also revealed ORFs for putative core proteins resembling pVII, p-mu and a partial ORF similar to pVI (hexon-associated protein) of HAdV-2 and HAdV-40. The results support the claim that HEV represents a distinct group of viruses within the genus Aviadenovirus . * Author for correspondence. Fax +1 703 231 3426. e-mail pierson@vt.edu Present address: Gen-Probe Inc., 9880 Campus Point Drive, San Diego, CA 92121-1589, USA. Present address: Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada. Received 1 August 1995; accepted 18 October 1995.