Use of anti-neurofilament antibody to identify paired-helical filaments in inclusion-body myositis

Paired‐helical filaments (PHFs) are an important diagnostic criterion of the inclusion‐body myositis (IBM) muscle biopsy; but, until now, their presence could be identified only by electronmicroscopy. In this report, we describe an easy immunocytochemical procedure, utilizing commercially available...

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Veröffentlicht in:	Annals of neurology 1996-03, Vol.39 (3), p.389-391
Hauptverfasser:	Askanas, Valerie, Alvarez, Renate B., Mirabella, Massimiliano, Engel, W. King
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal Biological and medical sciences Cytoplasm - ultrastructure Diseases of striated muscles. Neuromuscular diseases Humans Immunohistochemistry Medical sciences Muscle Fibers, Skeletal - ultrastructure Myositis, Inclusion Body - diagnosis Neurofilament Proteins - ultrastructure Neurology Vacuoles - ultrastructure
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Beschreibung
Zusammenfassung:	Paired‐helical filaments (PHFs) are an important diagnostic criterion of the inclusion‐body myositis (IBM) muscle biopsy; but, until now, their presence could be identified only by electronmicroscopy. In this report, we describe an easy immunocytochemical procedure, utilizing commercially available antibody, that enables reliable identification of muscle PHFs by light microscopy. This procedure greatly facilitates diagnosis of IBM.
ISSN:	0364-5134 1531-8249
DOI:	10.1002/ana.410390318