Direct detection of disomy in human sperm by the PRINS technique

We report the use of the PRimedIN Situ (PRINS) labelling technique for the direct estimation of disomy rates for various chromosomes in human sperm. The PRINS technique provides a rapid and reliable method for chromosome screening since specific labelling may be obtained in less than 2 h. In the pre...

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Veröffentlicht in:	Human genetics 1996, Vol.97 (1), p.21-25
Hauptverfasser:	PELLESTOR, F, QUENNESSON, I, COIGNET, L, GIRARDET, A, ANDREO, B, CHARLIEU, J. P
Format:	Artikel
Sprache:	eng
Schlagworte:	Aneuploidy Base Sequence Biological and medical sciences Chromosome Mapping Chromosomes, Human, Pair 12 Chromosomes, Human, Pair 18 Chromosomes, Human, Pair 2 Chromosomes, Human, Pair 5 Chromosomes, Human, Pair 9 Cytogenetics DNA Primers Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Human Humans In Situ Hybridization, Fluorescence Male Molecular Sequence Data Spermatozoa - cytology
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Zusammenfassung:	We report the use of the PRimedIN Situ (PRINS) labelling technique for the direct estimation of disomy rates for various chromosomes in human sperm. The PRINS technique provides a rapid and reliable method for chromosome screening since specific labelling may be obtained in less than 2 h. In the present study, the disomy rates of chromosomes 2, 5, 9, 12 and 18 were investigated. The incidences of disomy are similar for all these chromosomes, ranging from 0.27% to 0.33%. These findings suggest an equal distribution of aneuploidies among autosomes in human sperm.
ISSN:	0340-6717 1432-1203
DOI:	10.1007/BF00218827