Rapid isoelectric focusing in a vertical polyacrylamide minigel system

A rapid method is described for the resolution of proteins employing isoelectric focusing in a vertical polyacrylamide minigel system. Isoelectric focusing can be performed in only 3 h, utilizing low voltage, under either native conditions or denaturing conditions in the presence of 8 m urea. The pr...

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Veröffentlicht in:	Analytical biochemistry 1987-12, Vol.167 (2), p.290-294
Hauptverfasser:	Robertson, Eugene F., Dannelly, H.Kathleen, Malloy, Peter J., Reeves, Henry C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences electrophoresis Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Holoproteins isoelectric focusing Isoelectric Focusing - methods minigel Other proteins polyacrylamide gel protein Proteins Proteins - isolation & purification two-dimensional electrophoresis Urea
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Zusammenfassung:	A rapid method is described for the resolution of proteins employing isoelectric focusing in a vertical polyacrylamide minigel system. Isoelectric focusing can be performed in only 3 h, utilizing low voltage, under either native conditions or denaturing conditions in the presence of 8 m urea. The procedure permits the application of larger sample volumes containing more protein than other isoelectric focusing procedures, and provides the additional advantages of slab gels over tube gels for analytical purposes. The procedure is also well adapted for use in two-dimensional electrophoretic techniques, making it possible to complete a two-dimensional gel in 1 day.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(87)90166-7